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XB-ART-21567
Cell. February 25, 1994; 76 (4): 639-50.

Pericentrin, a highly conserved centrosome protein involved in microtubule organization.

Doxsey SJ , Stein P , Evans L , Calarco PD , Kirschner M .


Abstract
Antisera from scleroderma patients that react widely with centrosomes in plants and animals were used to isolate cDNAs encoding a novel centrosomal protein. The nucleotide sequence is consistent with a 7 kb mRNA and contains an open reading frame encoding a protein with a putative large coiled-coil domain flanked by noncoiled ends. Antisera recognize a 220 kd protein and stain centrosomes and acentriolar microtubule-organizing centers, where the protein is localized to the pericentriolar material (hence, the name pericentrin). Anti-pericentrin antibodies disrupt mitotic and meiotic divisions in vivo and block microtubule aster formation in Xenopus extracts, but do not block gamma-tubulin assembly or microtubule nucleation from mature centrosomes. These results suggest that pericentrin is a conserved integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays.

PubMed ID: 8124707
Article link: Cell.

Genes referenced: pcnt tubg1


References:
Archer, 1994, Pubmed, Xenbase


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