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Mech Dev March 1, 2005; 122 (3): 333-54.

Microarray-based identification of VegT targets in Xenopus.

Taverner NV , Kofron M , Shin Y , Kabitschke C , Gilchrist MJ , Wylie C , Cho KW , Heasman J , Smith JC .

The Xenopus T box family member VegT is expressed maternally in the vegetal hemisphere of the embryo. Mis-expression of VegT in prospective ectodermal tissue causes ectopic activation of mesodermal and endodermal markers, and ablation of VegT transcripts prevents proper formation of the mesendoderm, with the entire embryo developing as epidermis. These observations define VegT as a key initiator of mesendodermal development in the Xenopus embryo, and in an effort to understand how it exerts its effects we have used microarray analysis to compare gene expression in control animal caps with that in ectodermal tissue expressing an activated form of VegT. This procedure allowed the identification of 99 potential VegT targets, and we went on to study the expression patterns of these genes and then to ask, for those that are expressed in mesoderm or endoderm, which are direct targets of VegT. The putative regulatory regions of the resulting 14 genes were examined for T domain binding sites, and we also asked whether their expression is down-regulated in embryos in which VegT RNA is ablated. Finally, the functions of these genes were assayed by both over-expression and by use of antisense morpholino oligonucleotides. Our results provide new insights into the function of VegT during early Xenopus development.

PubMed ID: 15763211
Article link: Mech Dev
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: admp2 alpl aplnr atp1a1 best2 bix1.3 cdk5r1l cdx4 chrd.1 ckm copa cxcr4 cyp26a1 dhcr7 dhrs3 epha4 esr-5 exosc5 flrt3 foxb1 foxc1 fzd7 gdf3 ggt1 has2 hes5.7 hes7.2 hey1 kif26a krt18.1 krt8.1 lin28a mdn1 mid1ip1 mrpl24 msx1 myc myod1 nradd nrf1 pdlim5 pnhd post prickle1 pygm pzp rap2b rdh10 rhov rnf19a rpl24 rsl24d1 set slc12a3l slc16a6 slc2a1 slc6a14.2 snai1 sp5l spry1 stx6 tmod4 tp63 txnl4a utp4 vcan vegt ventx1.1 ventx1.2 wnt11b wnt5b XB5748694 [provisional:cdc42ep4] zic2 zswim4
Morpholinos: aplnr MO2 dhrs3 MO2 esr-5 MO1 hes7.2 MO1 hey1 MO1 pzp MO1 snai1 MO2

Article Images: [+] show captions