Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Determination of initiation of DNA replication before and after nuclear formation in Xenopus egg cell free extracts.
Kubota Y
,
Takisawa H
.
???displayArticle.abstract???
Xenopus egg extracts prepared before and after egg activation retain M- and S-phase specific activity, respectively. Staurosporine, a potent inhibitor of protein kinase, converted M-phase extracts into interphase-like extracts that were capable of forming nuclei upon the addition of sperm DNA. The nuclei formed in the staurosporine treated M-phase extract were incapable of replicating DNA, and they were unable to initiate replication upon the addition of S-phase extracts. Furthermore, replication was inhibited when the staurosporine-treated M-phase extract was added in excess to the staurosporine-treated S-phase extract before the addition of DNA. The membrane-depleted S-phase extract supported neither nuclear formation nor replication; however, preincubation of sperm DNA with these extracts allowed them to form replication-competent nuclei upon the addition of excess staurosporine-treated M-phase extract. These results demonstrate that positive factors in the S-phase extracts determined the initiation of DNA replication before nuclear formation, although these factors were unable to initiate replication after nuclear formation.
Adachi,
Identification of nuclear pre-replication centers poised for DNA synthesis in Xenopus egg extracts: immunolocalization study of replication protein A.
1992, Pubmed,
Xenbase
Adachi,
Identification of nuclear pre-replication centers poised for DNA synthesis in Xenopus egg extracts: immunolocalization study of replication protein A.
1992,
Pubmed
,
Xenbase
Bell,
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
1992,
Pubmed
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Blow,
Nuclei act as independent and integrated units of replication in a Xenopus cell-free DNA replication system.
1987,
Pubmed
,
Xenbase
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Blow,
Replication of purified DNA in Xenopus egg extract is dependent on nuclear assembly.
1990,
Pubmed
,
Xenbase
Bravo,
Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta.
,
Pubmed
Brewer,
The localization of replication origins on ARS plasmids in S. cerevisiae.
1987,
Pubmed
Diffley,
Protein-DNA interactions at a yeast replication origin.
1992,
Pubmed
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Felix,
A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity.
1989,
Pubmed
,
Xenbase
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Hirose,
Identification and subcellular localization of the polypeptide for chick DNA primase with a specific monoclonal antibody.
1988,
Pubmed
Huberman,
The in vivo replication origin of the yeast 2 microns plasmid.
1987,
Pubmed
Hutchison,
The control of DNA replication in a cell-free extract that recapitulates a basic cell cycle in vitro.
1988,
Pubmed
,
Xenbase
Jackson,
Replication occurs at a nucleoskeleton.
1986,
Pubmed
Krohne,
The nuclear lamins. A multigene family of proteins in evolution and differentiation.
1986,
Pubmed
,
Xenbase
Laskey,
S phase of the cell cycle.
1989,
Pubmed
Leno,
The nuclear membrane determines the timing of DNA replication in Xenopus egg extracts.
1991,
Pubmed
,
Xenbase
Leno,
The nuclear membrane prevents replication of human G2 nuclei but not G1 nuclei in Xenopus egg extract.
1992,
Pubmed
,
Xenbase
Lewin,
Driving the cell cycle: M phase kinase, its partners, and substrates.
1990,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lohka,
Roles of cytosol and cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs.
1984,
Pubmed
,
Xenbase
Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase
Meier,
The role of lamin LIII in nuclear assembly and DNA replication, in cell-free extracts of Xenopus eggs.
1991,
Pubmed
,
Xenbase
Newmeyer,
In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.
1986,
Pubmed
,
Xenbase
Newport,
Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.
1987,
Pubmed
,
Xenbase
Newport,
A lamin-independent pathway for nuclear envelope assembly.
1990,
Pubmed
,
Xenbase
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Penefsky,
Reversible binding of Pi by beef heart mitochondrial adenosine triphosphatase.
1977,
Pubmed
Pfaller,
Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro.
1991,
Pubmed
,
Xenbase
Prelich,
Functional identity of proliferating cell nuclear antigen and a DNA polymerase-delta auxiliary protein.
,
Pubmed
Rüegg,
Staurosporine, K-252 and UCN-01: potent but nonspecific inhibitors of protein kinases.
1989,
Pubmed
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Shinomiya,
Analysis of chromosomal replicons in early embryos of Drosophila melanogaster by two-dimensional gel electrophoresis.
1991,
Pubmed
Suzuki,
DNA primase-DNA polymerase alpha assembly from mouse FM3A cells. Purification of constituting enzymes, reconstitution, and analysis of RNA priming as coupled to DNA synthesis.
1989,
Pubmed
Tamaoki,
Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase.
1986,
Pubmed
Vaughn,
Replication initiates in a broad zone in the amplified CHO dihydrofolate reductase domain.
1990,
Pubmed
Vigers,
Regulation of nuclear envelope precursor functions during cell division.
1992,
Pubmed
,
Xenbase
Yamaguchi,
Distribution of PCNA in Drosophila embryo during nuclear division cycles.
1991,
Pubmed
