Lourim D , Krohne G .

	Figure I. Identification of Xenopus lamin Ln in oocyte nuclear envelopes. Nuclear envelopes from 50 oocytes (A-D), or follicle ceils derived from 25 oocytes (E) were salt washed and proteins electrophoretically separated by two-dimensional gel electrophoresis (first dimension, NEPHGE; second dimension, SDS-PAGE). Proteins were transferred to nitrocellulose filters and stained with Ponceau S to determine the position of the coelectrophoresed marker proteins actin, BSA, and PGK. The filters were probed with the antilamin Lm antibody 46F7 (A), the antilamin LII antibody X223 (B and E), or the antilamin Ln/Lm antibody X155 (D). The filter shown in A was reprobed with the antilamin LII antibody X223 (C). Arrowheads (A-C and F) mark the position of the quantitatively major isoelectric variant of oocyte lamin Ln. Brackets (D), indicate the isoelectric variants of L~ and Lm recognized by the antibody X155.
	Figure 2. Immunolocalization of lamin La in ovary. Indirect immunofluorescence microscopy on Xenopus ovary sections after incubation with antilamin Lm antibody 46F7 (A) or antilamin Lu antibody X223 (B). Chromatin staining (A~ B') by HOECHST. Bar, 50um.
	Figure 3. EM immunolocalization of lamin L~ to the oocyte nuclear lamina. Isolated oocyte nuclear envelopes were incubated with antilamin LI~ antibody X223 followed by secondary antibodies conjugated to 10-nm colloidal gold. Gold particles are observed exclusively on the nucleoplasmic (N) side of the envelope. Nuclear pore complexes are marked by arrowheads. C, cytoplasmic side. Bar, 0.2 um.
	Figure 4. Immunolocalization of lamins in two-cell-stage embryos by indirect immunofluorescence microscopy. Squashed preparations of in vitro fertilized embryos halted in interphase at the two-cell stage by cycloheximide were incubated with antilamin Lm antibody 46F7 (A) or antilamin LI~ antibody X223 (B), followed by Texas red-conjugated secondary antibodies. Chromatin staining (.4' and B') by HOECHST. Bar, 50um.
	Figure 5. EM analysis of egg extract fractionated by ultracentrifugation. Representative examples of materials found in the S~oo Sup. (A), $2oo Cytosol (B), and $2oo Pellet (C) are shown. Bar, 0.2 um.
	Figure 6. Distribution of nuclear envelope proteins in egg extract fractionated by ultracentrifugation. Proteins from the $1o0 Sup. (lane 1 ), serial dilutions of $2oo Cytosol (lanes 2-5), and $20o Pellet (lanes 6-8) were separated by 11% SDS-PAGE. Proteins were transferred to nitrocellulose filters then probed with anti-gp62 antiserum (A), anti-p54 antiserum (B), antilamin L./L,I antibody X155 (C), and antilamin Lm antibody 46F7 (D). Units refers to the relative amount of St0o Sup. (1.0 U equals 2.4 #1 of Si0o Sup., lane 1) contained in the $20o Cytosol (lanes 2-5) and $20o Pellet (lanes 6-8) aliquots used for gel separation.
	Figure 7. Immunoabsorption of vesicles to magnetic beads coated with antilamin antibodies. EM analysis of vesicles from S~0o Sup. immunoabsorbed to magnetic beads coated with control goat anti-mouse antibody (A), antilamin L~I antibody X223 (B), or antilamin Lm antibody 46F7 (C; D, higher magnification image of 46F7-absorbed vesicles). Bars, 0.2 #m (A-C); 0.1 #m (D).
	Figure 8. Immunological analysis of lamins absorbed to antibody-coated magnetic beads. An aliquot of S~0o Sup.- (lane 1 ) and proteins absorbed to control (lane 2)-, antilamin Lm antibody 46F7 (lane 3)-, and antilamin Ln antibody X223 (lane 4)- coated magnetic beads were solubilized, separated by 8 % SDS-PAGE, then transferred to nitrocellulose filters for probing with the antilamin LII/Lm antibody X155. Arrowheads indicate the migration position of the antibody heavy chains (HC) and lamins Lm and Ln are noted on the right of the panel.
	Figure 9. EM immunolocalization of lamins Ln and Lm on membranes present in the $200 Pellet. Membranes were immobilized onto glass coverslips and incubated with the antibodies X223 (A-E) or 46F7 (F-L). Bound antibodies were visualized with secondary antibodies conjugated to 12-nm colloidal gold. Overviews A (X223) and F (46F7) are shown at identical magnifications. Arrows in the overviews (A and F) designate vesicles labeled with gold particles. Higher magnification images of antibody X223-1abeled vesicles (B-E) and 46FTlabeled vesicles (G-L) are also presented. Bars, 0.2 #m (A and F); 0.1 #m (B-E and G--L).

Bailer, Characterization of A 54-kD protein of the inner nuclear membrane: evidence for cell cycle-dependent interaction with the nuclear lamina. 1991, Pubmed

Bailer, Characterization of A 54-kD protein of the inner nuclear membrane: evidence for cell cycle-dependent interaction with the nuclear lamina. 1991, Pubmed
Beams, Effects of ultracentrifugation on the interphase nucleus of somatic cells with special reference to the nuclear envelope-chromatin relationship. 1970, Pubmed
Beck, Isoprenylation is required for the processing of the lamin A precursor. 1990, Pubmed
Benavente, Involvement of nuclear lamins in postmitotic reorganization of chromatin as demonstrated by microinjection of lamin antibodies. 1986, Pubmed
Benavente, Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis. 1985, Pubmed , Xenbase
Burke, A cell free system to study reassembly of the nuclear envelope at the end of mitosis. 1986, Pubmed
Cordes, Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region. 1991, Pubmed , Xenbase
Dabauvalle, Spontaneous assembly of pore complex-containing membranes ("annulate lamellae") in Xenopus egg extract in the absence of chromatin. 1991, Pubmed , Xenbase
Dabauvalle, Identification of a soluble precursor complex essential for nuclear pore assembly in vitro. 1990, Pubmed , Xenbase
Dabauvalle, Assembly of nuclear pore complexes in Xenopus egg extract. 1991, Pubmed , Xenbase
Döring, Gene structure of nuclear lamin LIII of Xenopus laevis; a model for the evolution of IF proteins from a lamin-like ancestor. 1990, Pubmed , Xenbase
Furukawa, cDNA cloning of a germ cell specific lamin B3 from mouse spermatocytes and analysis of its function by ectopic expression in somatic cells. 1993, Pubmed
Gerace, The nuclear envelope lamina is reversibly depolymerized during mitosis. 1980, Pubmed
Gieffers, In vitro reconstitution of recombinant lamin A and a lamin A mutant lacking the carboxy-terminal tail. 1991, Pubmed , Xenbase
Glass, Lamins A and C bind and assemble at the surface of mitotic chromosomes. 1990, Pubmed
Hutchison, The control of DNA replication in a cell-free extract that recapitulates a basic cell cycle in vitro. 1988, Pubmed , Xenbase
Höger, Characterization of a second highly conserved B-type lamin present in cells previously thought to contain only a single B-type lamin. 1990, Pubmed , Xenbase
Höger, Immunolocalization of lamins in the thick nuclear lamina of human synovial cells. 1991, Pubmed
Krohne, The nuclear lamins. A multigene family of proteins in evolution and differentiation. 1986, Pubmed , Xenbase
Krohne, Nuclear lamin LI of Xenopus laevis: cDNA cloning, amino acid sequence and binding specificity of a member of the lamin B subfamily. 1987, Pubmed , Xenbase
Krohne, A monoclonal antibody against nuclear lamina proteins reveals cell type-specificity in Xenopus laevis. 1984, Pubmed , Xenbase
Kyhse-Andersen, Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. 1984, Pubmed
Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. 1970, Pubmed
Laskey, Assembly of the cell nucleus. 1990, Pubmed
Lebel, Lamins A and C appear during retinoic acid-induced differentiation of mouse embryonal carcinoma cells. 1987, Pubmed
Lebkowski, Non-histone proteins and long-range organization of HeLa interphase DNA. 1982, Pubmed
Lehner, Differential expression of nuclear lamin proteins during chicken development. 1987, Pubmed , Xenbase
Lourim, Expression of wild-type and nuclear localization-deficient human lamin A in chick myogenic cells. 1992, Pubmed
Lourim, Expression of nuclear lamin A and muscle-specific proteins in differentiating muscle cells in ovo and in vitro. 1989, Pubmed
Ludérus, Binding of matrix attachment regions to lamin B1. 1992, Pubmed
McKeon, Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins. , Pubmed
Meier, The role of lamin LIII in nuclear assembly and DNA replication, in cell-free extracts of Xenopus eggs. 1991, Pubmed , Xenbase
Newport, A lamin-independent pathway for nuclear envelope assembly. 1990, Pubmed , Xenbase
Newport, Characterization of the membrane binding and fusion events during nuclear envelope assembly using purified components. 1992, Pubmed , Xenbase
Nigg, The nuclear envelope. 1989, Pubmed
O'Farrell, High resolution two-dimensional electrophoresis of basic as well as acidic proteins. 1977, Pubmed
Peter, Cloning and sequencing of cDNA clones encoding chicken lamins A and B1 and comparison of the primary structures of vertebrate A- and B-type lamins. 1989, Pubmed
Röber, Differential timing of nuclear lamin A/C expression in the various organs of the mouse embryo and the young animal: a developmental study. 1989, Pubmed
Schatten, Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchins. 1985, Pubmed
Stewart, Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B. 1987, Pubmed
Stick, The gene structure of Xenopus nuclear lamin A: a model for the evolution of A-type from B-type lamins by exon shuffling. 1992, Pubmed , Xenbase
Stick, The fates of chicken nuclear lamin proteins during mitosis: evidence for a reversible redistribution of lamin B2 between inner nuclear membrane and elements of the endoplasmic reticulum. 1988, Pubmed
Stick, cDNA cloning of the developmentally regulated lamin LIII of Xenopus laevis. 1988, Pubmed , Xenbase
Stick, Changes in the nuclear lamina composition during early development of Xenopus laevis. 1985, Pubmed , Xenbase
Ulitzur, Lamin activity is essential for nuclear envelope assembly in a Drosophila embryo cell-free extract. 1992, Pubmed
Vigers, Regulation of nuclear envelope precursor functions during cell division. 1992, Pubmed , Xenbase
Vigers, A distinct vesicle population targets membranes and pore complexes to the nuclear envelope in Xenopus eggs. 1991, Pubmed , Xenbase
Vorburger, Modification of nuclear lamin proteins by a mevalonic acid derivative occurs in reticulocyte lysates and requires the cysteine residue of the C-terminal CXXM motif. 1989, Pubmed
Weber, Maturation of nuclear lamin A involves a specific carboxy-terminal trimming, which removes the polyisoprenylation site from the precursor; implications for the structure of the nuclear lamina. 1989, Pubmed
Wilson, A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro. 1988, Pubmed , Xenbase
Wolin, A new lamin in Xenopus somatic tissues displays strong homology to human lamin A. 1987, Pubmed , Xenbase
Zewe, Gene structure and chromosomal localization of the murine lamin B2 gene. 1991, Pubmed , Xenbase