Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Proc Natl Acad Sci U S A. March 22, 2005; 102 (12): 4318-23.

A role for the anaphase-promoting complex inhibitor Emi2/XErp1, a homolog of early mitotic inhibitor 1, in cytostatic factor arrest of Xenopus eggs.

Tung JJ , Hansen DV , Ban KH , Loktev AV , Summers MK , Adler JR , Jackson PK .

Unfertilized vertebrate eggs are arrested in metaphase of meiosis II with high cyclin B/Cdc2 activity to prevent parthenogenesis. Until fertilization, exit from metaphase is blocked by an activity called cytostatic factor (CSF), which stabilizes cyclin B by inhibiting the anaphase-promoting complex (APC) ubiquitin ligase. The APC inhibitor early mitotic inhibitor 1 (Emi1) was recently found to be required for maintenance of CSF arrest. We show here that exogenous Emi1 is unstable in CSF-arrested Xenopus eggs and is destroyed by the SCF(betaTrCP) ubiquitin ligase, suggesting that endogenous Emi1, an apparent 44-kDa protein, requires a stabilizing factor. However, anti-Emi1 antibodies crossreact with native Emi2/Erp1/FBXO43, a homolog of Emi1 and conserved APC inhibitor. Emi2 is stable in CSF-arrested eggs, is sufficient to prevent CSF release, and is rapidly degraded in a Polo-like kinase 1-dependent manner in response to calcium-mediated egg activation. These results identify Emi2 as a candidate CSF maintenance protein.

PubMed ID: 15753281
PMC ID: PMC552977
Article link: Proc Natl Acad Sci U S A.
Grant support: 5T32 CA09302-27 NCI NIH HHS , GM54811 NIGMS NIH HHS , R01 GM60439 NIGMS NIH HHS , R01 GM054811 NIGMS NIH HHS , T32 CA009302 NCI NIH HHS , T32 CA009151 NCI NIH HHS , R01 GM060439 NIGMS NIH HHS

Genes referenced: fbxo43 fbxo5

External Resources:

Bhatt, 1999, Pubmed, Xenbase[+]

Xenbase: The Xenopus laevis and X. tropicalis resource.
Version: 4.9.0
Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556