Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1993 Jul 15;9014:6557-61.
Show Gene links
Show Anatomy links
Binding and transcriptional activation of the promoter for the neural cell adhesion molecule by HoxC6 (Hox-3.3).
Jones FS
,
Holst BD
,
Minowa O
,
De Robertis EM
,
Edelman GM
.
???displayArticle.abstract???
Scores of homeobox gene-encoded transcription factors are expressed in a definite spatiotemporal pattern during embryogenesis and regulate a series of as yet unidentified target genes to help coordinate the morphogenetic process. We have suggested that homeobox gene products modulate the expression of adhesion molecule genes and have shown in cotransfection experiments that the promoters for the neural cell adhesion molecule (N-CAM) and cytotactin/tenascin genes respond to cues from different homeobox-containing genes. In this study, we show that the HoxC6 (Hox-3.3)-encoded homeoprotein binds to a DNA sequence in the N-CAM promoter CCTAATTATTAA, designated homeodomain binding site I (HBS-I). To test whether HoxC6 regulated N-CAM promoter activity, we cotransfected the Long and Short reading frame variants of Xenopus HoxC6 (CMV-HoxC6-L and CMV-HoxC6-S) driven by the human cytomegalovirus (CMV) promoter together with a chloramphenicol acetyltransferase (CAT) reporter gene driven by the mouse N-CAM promoter (N-CAM-Pro-CAT). Cotransfection of NIH 3T3 cells with either of the CMV-HoxC6 expression vectors stimulated N-CAM promoter-driven CAT expression. A 47-bp region from the N-CAM promoter that included HBS-I and an adjacent potential HBS, HBS-II, conferred HoxC6 regulation on a simian virus 40 minimal promoter. HBS-I was sufficient for transactivation of the minimal promoter by CMV-HoxC6-S. However, transcriptional activation by CMV-HoxC6-L required both HBS-I and HBS-II, inasmuch as mutation of either HBS-I, HBS-II, or both motifs abolished the response. These studies suggest that HBS-I is a target site for binding and transcriptional control of the N-CAM promoter by homeoproteins, although accessory DNA sequences (such as HBS-II) may also be required. Together with previous studies, these results support the notion that N-CAM gene expression may be controlled by different combinations of homeoproteins that appear in a place-dependent manner during embryogenesis.
Akam,
Hox and HOM: homologous gene clusters in insects and vertebrates.
1989, Pubmed
Akam,
Hox and HOM: homologous gene clusters in insects and vertebrates.
1989,
Pubmed
Ananthan,
Synergistic activation of transcription is mediated by the N-terminal domain of Drosophila fushi tarazu homeoprotein and can occur without DNA binding by the protein.
1993,
Pubmed
Andrew,
Downstream of the homeotic genes.
1992,
Pubmed
Bloch-Gallego,
Antennapedia homeobox peptide enhances growth and branching of embryonic chicken motoneurons in vitro.
1993,
Pubmed
Carrasco,
Cloning of an X. laevis gene expressed during early embryogenesis coding for a peptide region homologous to Drosophila homeotic genes.
1984,
Pubmed
,
Xenbase
Cho,
Differential utilization of the same reading frame in a Xenopus homeobox gene encodes two related proteins sharing the same DNA-binding specificity.
1988,
Pubmed
,
Xenbase
Chuong,
Gradients of homeoproteins in developing feather buds.
1990,
Pubmed
Desplan,
The sequence specificity of homeodomain-DNA interaction.
1988,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Edelman,
Cytotactin: a morphoregulatory molecule and a target for regulation by homeobox gene products.
1992,
Pubmed
Edelman,
Cell adhesion molecules: implications for a molecular histology.
1991,
Pubmed
Edelman,
Morphoregulation.
1992,
Pubmed
Galang,
Cooperative DNA binding of the highly conserved human Hox 2.1 homeodomain gene product.
1992,
Pubmed
Gaunt,
Mouse Hox-3.4: homeobox sequence and embryonic expression patterns compared with other members of the Hox gene network.
1990,
Pubmed
,
Xenbase
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Gould,
Connectin, a target of homeotic gene control in Drosophila.
1992,
Pubmed
Gould,
Targets of homeotic gene control in Drosophila.
1990,
Pubmed
Graham,
The murine Hox-2 genes display dynamic dorsoventral patterns of expression during central nervous system development.
1991,
Pubmed
Gurdon,
Embryonic induction--molecular prospects.
1987,
Pubmed
Han,
Synergistic activation and repression of transcription by Drosophila homeobox proteins.
1989,
Pubmed
Hirsch,
Identification of positive and negative regulatory elements governing cell-type-specific expression of the neural cell adhesion molecule gene.
1990,
Pubmed
Hortsch,
Cell and substrate adhesion molecules in Drosophila.
1991,
Pubmed
Joliot,
Antennapedia homeobox peptide regulates neural morphogenesis.
1991,
Pubmed
Jones,
Cell adhesion molecules as targets for Hox genes: neural cell adhesion molecule promoter activity is modulated by cotransfection with Hox-2.5 and -2.4.
1992,
Pubmed
,
Xenbase
Jones,
Activation of the cytotactin promoter by the homeobox-containing gene Evx-1.
1992,
Pubmed
Lewis,
A gene complex controlling segmentation in Drosophila.
1978,
Pubmed
McGinnis,
Homeobox genes and axial patterning.
1992,
Pubmed
Nose,
Connectin: a homophilic cell adhesion molecule expressed on a subset of muscles and the motoneurons that innervate them in Drosophila.
1992,
Pubmed
Oliver,
Differential antero-posterior expression of two proteins encoded by a homeobox gene in Xenopus and mouse embryos.
1988,
Pubmed
,
Xenbase
Reichardt,
Extracellular matrix molecules and their receptors: functions in neural development.
1991,
Pubmed
Salser,
Activation of a C. elegans Antennapedia homologue in migrating cells controls their direction of migration.
1992,
Pubmed
Scott,
The structure and function of the homeodomain.
1989,
Pubmed
Scott,
Vertebrate homeobox gene nomenclature.
1992,
Pubmed
Sonderegger,
Regulation of axonal growth in the vertebrate nervous system by interactions between glycoproteins belonging to two subgroups of the immunoglobulin superfamily.
1992,
Pubmed
Takeichi,
Cadherin cell adhesion receptors as a morphogenetic regulator.
1991,
Pubmed
Treisman,
The homeodomain: a new face for the helix-turn-helix?
1992,
Pubmed
Wright,
Interference with function of a homeobox gene in Xenopus embryos produces malformations of the anterior spinal cord.
1989,
Pubmed
,
Xenbase
