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Preferential and asymmetric interaction of linker histones with 5S DNA in the nucleosome.
Hayes JJ
,
Wolffe AP
.
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We establish that linker histones H1 and H5 bind preferentially to a Xenopus borealis somatic 5S RNA gene associated with an octamer of core histones rather than to naked 5S DNA. This preferential binding requires free linker DNA to either side of the nucleosome core. Incorporation of a single linker histone molecule into the nucleosome protects an additional 20 bp of linker DNA from micrococcal nuclease digestion. This additional DNA is asymmetrically distributed with respect to the nucleosome core. Incorporation of linker histones causes no change to the cleavage of DNA in the nucleosome by hydroxyl radical or DNase I.
Allan,
The structure of histone H1 and its location in chromatin.
1980, Pubmed
Allan,
The structure of histone H1 and its location in chromatin.
1980,
Pubmed
Arents,
The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix.
1991,
Pubmed
Ausio,
Histone hyperacetylation: its effects on nucleosome conformation and stability.
1986,
Pubmed
Boulikas,
Points of contact between histone H1 and the histone octamer.
1980,
Pubmed
Brand,
The properties of salt-extracted histone H1.
1981,
Pubmed
Buckle,
Site-directed mutagenesis studies on the binding of the globular domain of linker histone H5 to the nucleosome.
1992,
Pubmed
Camerini-Otero,
The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.
1976,
Pubmed
Caron,
Exchange of histone H1 between segments of chromatin.
1981,
Pubmed
Clark,
Salt-dependent co-operative interaction of histone H1 with linear DNA.
1986,
Pubmed
Dingwall,
High sequence specificity of micrococcal nuclease.
1981,
Pubmed
Dong,
DNA and protein determinants of nucleosome positioning on sea urchin 5S rRNA gene sequences in vitro.
1990,
Pubmed
Dong,
Nucleosome positioning is determined by the (H3-H4)2 tetramer.
1991,
Pubmed
Hansen,
Homogeneous reconstituted oligonucleosomes, evidence for salt-dependent folding in the absence of histone H1.
1989,
Pubmed
Hayes,
The structure of DNA in a nucleosome.
1990,
Pubmed
,
Xenbase
Hayes,
Histone contributions to the structure of DNA in the nucleosome.
1991,
Pubmed
,
Xenbase
Hayes,
Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
1992,
Pubmed
,
Xenbase
Meersseman,
Chromatosome positioning on assembled long chromatin. Linker histones affect nucleosome placement on 5 S rDNA.
1991,
Pubmed
Nelson,
Reassociation of histone H1 with nucleosomes.
1979,
Pubmed
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Renz,
Preferential and cooperative binding of histone I to chromosomal mammalian DNA.
1975,
Pubmed
Richmond,
Structure of the nucleosome core particle at 7 A resolution.
,
Pubmed
Satchwell,
Asymmetry and polarity of nucleosomes in chicken erythrocyte chromatin.
1989,
Pubmed
Simon,
A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.
1979,
Pubmed
Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed
Simpson,
Nucleosome positioning: occurrence, mechanisms, and functional consequences.
1991,
Pubmed
Simpson,
Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones.
1978,
Pubmed
Singer,
Studies on the interaction of H1 histone with superhelical DNA: characterization of the recognition and binding regions of H1 histones.
1976,
Pubmed
Staynov,
Footprinting of linker histones H5 and H1 on the nucleosome.
1988,
Pubmed
Tatchell,
Reconstitution of chromatin core particles.
1977,
Pubmed
Todd,
Overall pathway of mononucleosome production.
1979,
Pubmed
Tullius,
Hydroxyl radical footprinting: a high-resolution method for mapping protein-DNA contacts.
1987,
Pubmed
Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase
Yao,
Direct detection of linker DNA bending in defined-length oligomers of chromatin.
1990,
Pubmed
Yao,
Linker DNA bending induced by the core histones of chromatin.
1991,
Pubmed
