Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1993 Mar 01;133:1480-8. doi: 10.1128/mcb.13.3.1480-1488.1993.
Show Gene links
Show Anatomy links
Mitotic arrest caused by the amino terminus of Xenopus cyclin B2.
van der Velden HM
,
Lohka MJ
.
???displayArticle.abstract???
Progression through mitosis requires the inactivation of the protein kinase activity of the p34cdc2-cyclin complex by a mechanism involving the degradation of cyclin. We have examined the stability in Xenopus egg extracts of radiolabeled Xenopus or sea urchin B-type cyclins synthesized in reticulocyte lysates. Xenopus cyclin B2 and sea urchin cyclin B were stable in metaphase extracts from unfertilized eggs but were specifically degraded following addition of Ca2+ to the extracts. The degradation of either cyclin was inhibited by the addition of an excess of unlabeled Xenopus cyclin B2 but not by the addition of a number of control proteins. A truncated protein containing only the amino terminus of Xenopus cyclin B2, including sequences known to be essential for cyclin degradation in other species, also inhibited cyclin degradation, even though the truncated protein was stable in extracts following Ca2+ addition. The addition of the truncated protein did not stimulate histone H1 kinase activity in extracts but prevented the loss of H1 kinase activity that normally follows Ca2+ addition to metaphase extracts. When the amino-terminal fragment was added to extracts capable of several cell cycles in vitro, progression through the first mitosis was inhibited and elevated histone H1 kinase activity was maintained. These results indicate that although the amino terminus of cyclin does not contain all of the information necessary for cyclin destruction, it is capable of interacting with components of the cyclin destruction pathway and thereby preventing the degradation of full-length cyclins.
Chamberlain,
Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
1979, Pubmed
Chamberlain,
Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
1979,
Pubmed
Daar,
The ras oncoprotein and M-phase activity.
1991,
Pubmed
,
Xenbase
Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed
Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
Gallant,
Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells.
1992,
Pubmed
,
Xenbase
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Ghiara,
A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis.
1991,
Pubmed
Glotzer,
Cyclin is degraded by the ubiquitin pathway.
1991,
Pubmed
,
Xenbase
Gould,
Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function.
1991,
Pubmed
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Hager,
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
1980,
Pubmed
Hershko,
Methylated ubiquitin inhibits cyclin degradation in clam embryo extracts.
1991,
Pubmed
Hunt,
The requirements for protein synthesis and degradation, and the control of destruction of cyclins A and B in the meiotic and mitotic cell cycles of the clam embryo.
1992,
Pubmed
Hunt,
Cyclins and their partners: from a simple idea to complicated reality.
1991,
Pubmed
Izumi,
Phosphorylation of Xenopus cyclins B1 and B2 is not required for cell cycle transitions.
1991,
Pubmed
,
Xenbase
Kumagai,
The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system.
1991,
Pubmed
,
Xenbase
Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
Lew,
Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast.
1991,
Pubmed
Lewin,
Driving the cell cycle: M phase kinase, its partners, and substrates.
1990,
Pubmed
Loetscher,
The C terminus of mouse ornithine decarboxylase confers rapid degradation on dihydrofolate reductase. Support for the pest hypothesis.
1991,
Pubmed
,
Xenbase
Lohka,
Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts.
1985,
Pubmed
,
Xenbase
Lorca,
An okadaic acid-sensitive phosphatase negatively controls the cyclin degradation pathway in amphibian eggs.
1991,
Pubmed
,
Xenbase
Luca,
Both cyclin A delta 60 and B delta 97 are stable and arrest cells in M-phase, but only cyclin B delta 97 turns on cyclin destruction.
1991,
Pubmed
,
Xenbase
Luca,
Control of programmed cyclin destruction in a cell-free system.
1989,
Pubmed
Matsushime,
Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle.
1991,
Pubmed
Meyerhof,
Ca and Mg control of cytostatic factors from Rana pipiens oocytes which cause metaphase and cleavage arrest.
1977,
Pubmed
Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase
Minshull,
The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle.
1990,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Murray,
Dominoes and clocks: the union of two views of the cell cycle.
1989,
Pubmed
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Pines,
Human cyclin A is adenovirus E1A-associated protein p60 and behaves differently from cyclin B.
1990,
Pubmed
Pines,
p34cdc2: the S and M kinase?
1990,
Pubmed
Pines,
Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport.
1991,
Pubmed
Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed
Pines,
Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs.
1987,
Pubmed
,
Xenbase
Reed,
G1-specific cyclins: in search of an S-phase-promoting factor.
1991,
Pubmed
Roy,
Activation of p34cdc2 kinase by cyclin A.
1991,
Pubmed
,
Xenbase
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Shibuya,
Fractionation of cytostatic factors from cytosols of amphibian eggs.
1989,
Pubmed
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Westendorf,
The role of cyclin B in meiosis I.
1989,
Pubmed
,
Xenbase
Wittenberg,
G1-specific cyclins of S. cerevisiae: cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinase.
1990,
Pubmed
