XB-ART-22886
J Cell Biol
1993 Feb 01;1203:613-24.
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Mitotic repression of transcription in vitro.
Hartl P
,
Gottesfeld J
,
Forbes DJ
.
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A normal consequence of mitosis in eukaryotes is the repression of transcription. Using Xenopus egg extracts shifted to a mitotic state by the addition of purified cyclin, we have for the first time been able to reproduce a mitotic repression of transcription in vitro. Active RNA polymerase III transcription is observed in interphase extracts, but strongly repressed in extracts converted to mitosis. With the topoisomerase II inhibitor VM-26, we demonstrate that this mitotic repression of RNA polymerase III transcription does not require normal chromatin condensation. Similarly; in vitro mitotic repression of transcription does not require the presence of nucleosome structure or involve a general repressive chromatin-binding protein, as inhibition of chromatin formation with saturating amounts of non-specific DNA has no effect on repression. Instead, the mitotic repression of transcription appears to be due to phosphorylation of a component of the transcription machinery by a mitotic protein kinase, either cdc2 kinase and/or a kinase activated by it. Mitotic repression of RNA polymerase III transcription is observed both in complete mitotic cytosol and when a kinase-enriched mitotic fraction is added to a highly simplified 5S RNA transcription reaction. We present evidence that, upon depletion of cdc2 kinase, a secondary protein kinase activity remains and can mediate this in vitro mitotic repression of transcription.
???displayArticle.pubmedLink??? 8381119
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Species referenced: Xenopus laevis
Genes referenced: cdk1
References [+] :
Adachi,
Chromosome assembly in vitro: topoisomerase II is required for condensation.
1991, Pubmed,
Xenbase
Adachi, Chromosome assembly in vitro: topoisomerase II is required for condensation. 1991, Pubmed , Xenbase
Almouzni, Transcription complex disruption caused by a transition in chromatin structure. 1991, Pubmed , Xenbase
Almouzni, Competition between transcription complex assembly and chromatin assembly on replicating DNA. 1990, Pubmed , Xenbase
Binétruy, Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. 1991, Pubmed
Boyle, Activation of protein kinase C decreases phosphorylation of c-Jun at sites that negatively regulate its DNA-binding activity. 1991, Pubmed
Bradbury, Reversible histone modifications and the chromosome cell cycle. 1992, Pubmed
Bradbury, Control of cell division by very lysine rich histone (F1) phosphorylation. 1974, Pubmed
Brizuela, p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase. 1987, Pubmed
Brown, The role of stable complexes that repress and activate eucaryotic genes. 1984, Pubmed , Xenbase
Carrara, Deletion of the 3' half of the yeast tRNA-Leu3 gene does not abolish promotor function in vitro. 1981, Pubmed , Xenbase
Chen, Nonintercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA topoisomerase II. 1984, Pubmed
Cisek, Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2. 1989, Pubmed
Cohen, The structure and regulation of protein phosphatases. 1989, Pubmed
Comai, The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1. 1992, Pubmed
Corden, Tails of RNA polymerase II. 1990, Pubmed
Cormack, The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells. 1992, Pubmed
Dilworth, Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts. 1987, Pubmed , Xenbase
Draetta, Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF. 1989, Pubmed
Dunphy, Mitosis-inducing factors are present in a latent form during interphase in the Xenopus embryo. 1988, Pubmed , Xenbase
Dunphy, The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis. 1988, Pubmed , Xenbase
Edgar, Parameters controlling transcriptional activation during early Drosophila development. 1986, Pubmed
Edgar, Cell cycle control by the nucleo-cytoplasmic ratio in early Drosophila development. 1986, Pubmed
Fang, Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes. 1991, Pubmed , Xenbase
Felsenfeld, Structure of the 30 nm chromatin fiber. 1986, Pubmed
Felsenfeld, Chromatin as an essential part of the transcriptional mechanism. 1992, Pubmed
Felts, Transcription factor requirements for in vitro formation of transcriptionally competent 5S rRNA gene chromatin. 1990, Pubmed
Fink, Synthesis of transfer RNA during the synchronous nuclear division cycle in Physarum polycephalum. 1977, Pubmed
Fletcher, Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene. 1987, Pubmed
Forbes, Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs. 1983, Pubmed , Xenbase
Fotedar, Association of p34cdc2 with replicating DNA. 1991, Pubmed
Gautier, Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+. 1988, Pubmed , Xenbase
Gazit, Active genes are sensitive to deoxyribonuclease I during metaphase. 1982, Pubmed
Geiduschek, Transcription by RNA polymerase III. 1988, Pubmed
Glotzer, Cyclin is degraded by the ubiquitin pathway. 1991, Pubmed , Xenbase
Gottesfeld, Assembly of transcriptionally active 5S RNA gene chromatin in vitro. 1982, Pubmed , Xenbase
Gottesfeld, Novobiocin inhibits RNA polymerase III transcription in vitro by a mechanism distinct from DNA topoisomerase II. 1986, Pubmed
Heck, In vivo phosphorylation of the 170-kDa form of eukaryotic DNA topoisomerase II. Cell cycle analysis. 1989, Pubmed
Hirano, Cell cycle control of higher-order chromatin assembly around naked DNA in vitro. 1991, Pubmed , Xenbase
Johnson, Ribonucleic acid and protein synthesis in mitotic HeLa cells. 1965, Pubmed
Johnston, The yeast DNA polymerase I transcript is regulated in both the mitotic cell cycle and in meiosis and is also induced after DNA damage. 1987, Pubmed
Kassavetis, Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes. 1989, Pubmed
Keller, Additional intragenic promoter elements of the Xenopus 5S RNA genes upstream from the TFIIIA-binding site. 1990, Pubmed , Xenbase
Kerem, Mapping of DNAase I sensitive regions on mitotic chromosomes. 1984, Pubmed
Kimelman, The events of the midblastula transition in Xenopus are regulated by changes in the cell cycle. 1987, Pubmed , Xenbase
Kornberg, Irresistible force meets immovable object: transcription and the nucleosome. 1991, Pubmed
Labbe, Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation. 1989, Pubmed , Xenbase
Laskey, Assembly of SV40 chromatin in a cell-free system from Xenopus eggs. 1977, Pubmed , Xenbase
Laskey, Assembly of the cell nucleus. 1990, Pubmed
Laybourn, Role of nucleosomal cores and histone H1 in regulation of transcription by RNA polymerase II. 1991, Pubmed
Lewin, Driving the cell cycle: M phase kinase, its partners, and substrates. 1990, Pubmed
Lewis, Interphase nuclear matrix and metaphase scaffolding structures. 1984, Pubmed
Liu, DNA topoisomerase poisons as antitumor drugs. 1989, Pubmed
Lohka, Induction of nuclear envelope breakdown, chromosome condensation, and spindle formation in cell-free extracts. 1985, Pubmed , Xenbase
Lohka, Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components. 1983, Pubmed , Xenbase
Mattoccia, Site selection by the tRNA splicing endonuclease of Xenopus laevis. 1988, Pubmed , Xenbase
Miake-Lye, Induction of early mitotic events in a cell-free system. 1985, Pubmed , Xenbase
Millstein, Control of gene expression in eukaryotic cells: lessons from class III genes. 1989, Pubmed , Xenbase
Minshull, Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis. 1989, Pubmed , Xenbase
Moreno, Substrates for p34cdc2: in vivo veritas? 1990, Pubmed
Murray, The role of cyclin synthesis and degradation in the control of maturation promoting factor activity. 1989, Pubmed , Xenbase
Newport, Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes. 1987, Pubmed , Xenbase
Newport, Regulation of the cell cycle during early Xenopus development. 1984, Pubmed , Xenbase
Newport, The role of mitotic factors in regulating the timing of the midblastula transition in Xenopus. 1985, Pubmed , Xenbase
Newport, Nuclear reconstitution in vitro: stages of assembly around protein-free DNA. 1987, Pubmed , Xenbase
Otsuka, Ribonuclease "XlaI," an activity from Xenopus laevis oocytes that excises intervening sequences from yeast transfer ribonucleic acid precursors. 1981, Pubmed , Xenbase
PRESCOTT, Synthesis of RNA and protein during mitosis in mammalian tissue culture cells. 1962, Pubmed
Peterson, Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA. 1980, Pubmed , Xenbase
Pfaller, Assembly/disassembly of the nuclear envelope membrane: cell cycle-dependent binding of nuclear membrane vesicles to chromatin in vitro. 1991, Pubmed , Xenbase
Pines, p34cdc2: the S and M kinase? 1990, Pubmed
Razik, Pathways of nucleoprotein assembly on 5S RNA genes in a Xenopus oocyte S-150 extract. 1989, Pubmed , Xenbase
Roberge, The topoisomerase II inhibitor VM-26 induces marked changes in histone H1 kinase activity, histones H1 and H3 phosphorylation, and chromosome condensation in G2 phase and mitotic BHK cells. 1990, Pubmed
Roberts, Differential phosphorylation of the transcription factor Oct1 during the cell cycle. 1991, Pubmed
Schlissel, The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1. 1984, Pubmed , Xenbase
Schultz, Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters. 1992, Pubmed
Segil, Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity. 1991, Pubmed
Shermoen, Progression of the cell cycle through mitosis leads to abortion of nascent transcripts. 1991, Pubmed
Shimamura, Histone H1 represses transcription from minichromosomes assembled in vitro. 1989, Pubmed , Xenbase
Simanis, The cell cycle control gene cdc2+ of fission yeast encodes a protein kinase potentially regulated by phosphorylation. 1986, Pubmed
Simpson, Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure. 1985, Pubmed
Smythe, Coupling of mitosis to the completion of S phase in Xenopus occurs via modulation of the tyrosine kinase that phosphorylates p34cdc2. 1992, Pubmed , Xenbase
Smythe, Systems for the study of nuclear assembly, DNA replication, and nuclear breakdown in Xenopus laevis egg extracts. 1991, Pubmed , Xenbase
Solomon, Cyclin activation of p34cdc2. 1990, Pubmed , Xenbase
Toyoda, In vitro transcription by RNA polymerase II in extracts of Xenopus oocytes, eggs, and somatic cells. 1992, Pubmed , Xenbase
Uemura, DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe. 1987, Pubmed
Weintraub, Assembly and propagation of repressed and depressed chromosomal states. 1985, Pubmed
White, The CDC8 transcript is cell cycle regulated in yeast and is expressed coordinately with CDC9 and CDC21 at a point preceding histone transcription. 1987, Pubmed
White, A role for the TATA-box-binding protein component of the transcription factor IID complex as a general RNA polymerase III transcription factor. 1992, Pubmed
Wilson, A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro. 1988, Pubmed , Xenbase
Wolffe, Dominant and specific repression of Xenopus oocyte 5S RNA genes and satellite I DNA by histone H1. 1989, Pubmed , Xenbase
Wolffe, Differential 5S RNA gene expression in vitro. 1987, Pubmed , Xenbase
Wolffe, Negative supercoiling is not required for 5S RNA transcription in vitro. 1987, Pubmed , Xenbase
Wolffe, Developmental regulation of chromatin structure and function. 1991, Pubmed
Wood, Mitotic chromatin condensation in vitro using somatic cell extracts and nuclei with variable levels of endogenous topoisomerase II. 1990, Pubmed
Wu, Partial purification and characterization of the maturation-promoting factor from eggs of Xenopus laevis. 1980, Pubmed , Xenbase