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EMBO J
1993 Feb 01;122:573-83. doi: 10.1002/j.1460-2075.1993.tb05689.x.
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Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap.
Fischer U
,
Sumpter V
,
Sekine M
,
Satoh T
,
Lührmann R
.
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We have investigated the nuclear transport of U1 and U5 snRNPs by microinjection studies in oocytes from Xenopus laevis using snRNP particles prepared by reconstitution in vitro. Competition studies with snRNPs showed that the Sm core domain of U1 snRNPs contains a nuclear location signal that acts independently of the m3G cap. The transport of U1 snRNP can be blocked by saturation with competitor U1 snRNPs or by U5 snRNPs, which indicates that the signals on the respective Sm core domains interact with the same transport receptors. Further, by using a minimal U1 snRNP particle reconstituted in vitro and containing only the Sm core RNP domain and lacking stem-loops I to III of U1 RNA, we show that this is targeted actively to the nucleus, in spite of the absence of the m3G cap. This indicates that under certain conditions the NLS in the Sm core domain not only is an essential, but may also be a sufficient condition for nuclear targeting. We propose that the RNA structure of a given snRNP particle determines at least in part whether the particle's m3G cap is required for nuclear transport or can be dispensed with.
Adam,
Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import.
1991, Pubmed
Adam,
Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import.
1991,
Pubmed
Adam,
Identification of specific binding proteins for a nuclear location sequence.
1989,
Pubmed
Branlant,
U2 RNA shares a structural domain with U1, U4, and U5 RNAs.
1982,
Pubmed
Bringmann,
Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.
1983,
Pubmed
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Dingwall,
Nuclear targeting sequences--a consensus?
1991,
Pubmed
Dworetzky,
Translocation of RNA-coated gold particles through the nuclear pores of oocytes.
1988,
Pubmed
,
Xenbase
England,
3'-terminal labelling of RNA with T4 RNA ligase.
1978,
Pubmed
Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase
Fischer,
An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus.
1990,
Pubmed
,
Xenbase
Fischer,
Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport.
1991,
Pubmed
,
Xenbase
Garcia-Bustos,
Nuclear protein localization.
1991,
Pubmed
Goldfarb,
Synthetic peptides as nuclear localization signals.
,
Pubmed
,
Xenbase
Hamm,
The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal.
1990,
Pubmed
,
Xenbase
Kalderon,
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Kastner,
Electron microscopy of U1 small nuclear ribonucleoprotein particles: shape of the particle and position of the 5' RNA terminus.
1989,
Pubmed
Kastner,
Electron microscopy of small nuclear ribonucleoprotein (snRNP) particles U2 and U5: evidence for a common structure-determining principle in the major U snRNP family.
1990,
Pubmed
Konings,
Mutant U2 snRNAs of Xenopus which can form an altered higher order RNA structure are unable to enter the nucleus.
1987,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lanford,
Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal.
1986,
Pubmed
Lehmeier,
Evidence for three distinct D proteins, which react differentially with anti-Sm autoantibodies, in the cores of the major snRNPs U1, U2, U4/U6 and U5.
1990,
Pubmed
Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
Pubmed
Lerner,
Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
1981,
Pubmed
,
Xenbase
Liautard,
Structural organization of ribonucleoproteins containing small nuclear RNAs from HeLa cells. Proteins interact closely with a similar structural domain of U1, U2, U4 and U5 small nuclear RNAs.
1982,
Pubmed
Luhrmann,
Isolation and characterization of rabbit anti-m3 2,2,7G antibodies.
1982,
Pubmed
Lührmann,
Structure of spliceosomal snRNPs and their role in pre-mRNA splicing.
1990,
Pubmed
Mattaj,
Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins.
1985,
Pubmed
,
Xenbase
Mattaj,
Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding.
1986,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Michaud,
Multiple pathways in nuclear transport: the import of U2 snRNP occurs by a novel kinetic pathway.
1991,
Pubmed
,
Xenbase
Michaud,
Microinjected U snRNAs are imported to oocyte nuclei via the nuclear pore complex by three distinguishable targeting pathways.
1992,
Pubmed
,
Xenbase
Neuman de Vegvar,
Nucleocytoplasmic transport and processing of small nuclear RNA precursors.
1990,
Pubmed
,
Xenbase
Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase
Patton,
Reconstitution of the U1 small nuclear ribonucleoprotein particle.
1987,
Pubmed
Richardson,
Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.
1988,
Pubmed
,
Xenbase
Robbins,
Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
1991,
Pubmed
,
Xenbase
Silver,
How proteins enter the nucleus.
1991,
Pubmed
Singh,
Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA.
1989,
Pubmed
Sumpter,
In vitro reconstitution of U1 and U2 snRNPs from isolated proteins and snRNA.
1992,
Pubmed
Wersig,
Assembly and nuclear transport of the U4 and U4/U6 snRNPs.
1992,
Pubmed
,
Xenbase
