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Primary structure of an apical protein from Xenopus laevis that participates in amiloride-sensitive sodium channel activity.
Staub O
,
Verrey F
,
Kleyman TR
,
Benos DJ
,
Rossier BC
,
Kraehenbuhl JP
.
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High resistance epithelia express on their apical side an amiloride-sensitive sodium channel that controls sodium reabsorption. A cDNA was found to encode a 1,420-amino acid long polypeptide with no signal sequence, a putative transmembrane segment, and three predicted amphipathic alpha helices. A corresponding 5.2-kb mRNA was detected in Xenopus laevis kidney, intestine, and oocytes, with weak expression in stomach and eyes. An antibody directed against a fusion protein containing a COOH-terminus segment of the protein and an antiidiotypic antibody known to recognize the amiloride binding site of the epithelial sodium channel (Kleyman, T. R., J.-P. Kraehenbuhl, and S. A. Ernst. 1991. J. Biol. Chem. 266:3907-3915) immunoprecipitated a similar protein complex from [35S]methionine-labeled and from apically radioiodinated Xenopus laevis kidney-derived A6 cells. A single integral of 130-kD protein was recovered from samples reduced with DTT. The antibody also cross-reacted by ELISA with the putative amiloride-sensitive sodium channel isolated from A6 cells (Benos, D. J., G. Saccomani, and S. Sariban-Sohraby. 1987. J. Biol. Chem. 262:10613-10618). Although the protein is translated, cRNA injected into oocytes did not reconstitute amiloride-sensitive sodium transport, while antisense RNA or antisense oligodeoxynucleotides specific for two distinct sequences of the cloned cDNA inhibited amiloride-sensitive sodium current induced by injection of A6 cell mRNA. We propose that the cDNA encodes an apical plasma membrane protein that plays a role in the functional expression of the amiloride-sensitive epithelial sodium channel. It may represent a subunit of the Xenopus laevis sodium channel or a regulatory protein essential for sodium channel function.
Ausiello,
Purified epithelial Na+ channel complex contains the pertussis toxin-sensitive G alpha i-3 protein.
1992, Pubmed,
Xenbase
Ausiello,
Purified epithelial Na+ channel complex contains the pertussis toxin-sensitive G alpha i-3 protein.
1992,
Pubmed
,
Xenbase
Barbry,
[3H]phenamil binding protein of the renal epithelium Na+ channel. Purification, affinity labeling, and functional reconstitution.
1990,
Pubmed
Barbry,
Human kidney amiloride-binding protein: cDNA structure and functional expression.
1990,
Pubmed
Barbry,
Purification and subunit structure of the [3H]phenamil receptor associated with the renal apical Na+ channel.
1987,
Pubmed
Benos,
The epithelial sodium channel. Subunit number and location of the amiloride binding site.
1987,
Pubmed
Biggers,
Mammalian blastocyst: transport functions in a developing epithelium.
1988,
Pubmed
Colman,
Antisense strategies in cell and developmental biology.
1990,
Pubmed
Garty,
Characteristics and regulatory mechanisms of the amiloride-blockable Na+ channel.
1988,
Pubmed
Geering,
Subunit assembly and functional maturation of Na,K-ATPase.
1990,
Pubmed
Geering,
Membrane insertion of alpha- and beta-subunits of Na+,K+-ATPase.
1985,
Pubmed
,
Xenbase
George,
Functional expression of the amiloride-sensitive sodium channel in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Hamilton,
Single-channel recordings from two types of amiloride-sensitive epithelial Na+ channels.
1986,
Pubmed
Hediger,
Expression cloning and cDNA sequencing of the Na+/glucose co-transporter.
,
Pubmed
,
Xenbase
Hinton,
Expression of amiloride-blockable sodium channels in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Horisberger,
Coexpression of alpha 1 with putative beta 3 subunits results in functional Na+/K+ pumps in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Hubbard,
Externally disposed plasma membrane proteins. I. Enzymatic iodination of mouse L cells.
1975,
Pubmed
Jentsch,
Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Kleyman,
Characterization and cellular localization of the epithelial Na+ channel. Studies using an anti-Na+ channel antibody raised by an antiidiotypic route.
1991,
Pubmed
,
Xenbase
Kleyman,
The cellular pool of Na+ channels in the amphibian cell line A6 is not altered by mineralocorticoids. Analysis using a new photoactive amiloride analog in combination with anti-amiloride antibodies.
1989,
Pubmed
,
Xenbase
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Krattenmacher,
Ca-sensitive sodium absorption in the colon of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Kroll,
Selective expression of an amiloride-inhibitable Na+ conductance from mRNA of respiratory epithelium in Xenopus laevis oocytes.
1989,
Pubmed
,
Xenbase
Lewis,
Urinary proteases degrade epithelial sodium channels.
1991,
Pubmed
Machen,
Na+ transport by mammalian stomach.
1978,
Pubmed
Marston,
Solubilization and activation of recombinant calf prochymosin from Escherichia coli.
1985,
Pubmed
Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
Xenbase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Noda,
Primary structure of Electrophorus electricus sodium channel deduced from cDNA sequence.
,
Pubmed
Palmer,
Expression of epithelial Na channels in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Palmer,
Amiloride-sensitive Na channels from the apical membrane of the rat cortical collecting tubule.
1986,
Pubmed
Peitsch,
Localization and molecular modelling of the membrane-inserted domain of the ninth component of human complement and perforin.
1990,
Pubmed
Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sariban-Sohraby,
Single-channel behavior of a purified epithelial Na+ channel subunit that binds amiloride.
1992,
Pubmed
Sariban-Sohraby,
Detergent solubilization, functional reconstitution, and partial purification of epithelial amiloride-binding protein.
1986,
Pubmed
Shuttleworth,
Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs.
1988,
Pubmed
,
Xenbase
Smith,
Epithelial Na+ channels.
1991,
Pubmed
Sorscher,
Antibodies against purified epithelial sodium channel protein from bovine renal papilla.
1988,
Pubmed
Stanley,
Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.
1984,
Pubmed
Verrey,
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells.
1989,
Pubmed
,
Xenbase
Werner,
Cloning and expression of cDNA for a Na/Pi cotransport system of kidney cortex.
1991,
Pubmed
,
Xenbase
Zeiske,
Na+ channels and amiloride-induced noise in the mammalian colon epithelium.
1982,
Pubmed
