XB-ART-23191
Proc Natl Acad Sci U S A
November 15, 1992;
89
(22):
10955-9.
Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain.
Storck T
,
Schulte S
,
Hofmann K
,
Stoffel W
.
Abstract
Transport systems specific for L-glutamate and L-aspartate play an important role in the termination of neurotransmitter signals at excitatory synapses. We describe here the structure and function of a 66-kDa glycoprotein that was purified from rat
brain and identified as an L-glutamate/L-aspartate transporter (
GLAST). A
GLAST-specific cDNA clone was isolated from a rat
brain cDNA library. The cDNA insert encodes a polypeptide with 543 amino acid residues (59,697 Da). The amino acid sequence of
GLAST suggests a distinctive structure and membrane topology, with some conserved motifs also present in prokaryotic glutamate transporters. The transporter function has been verified by amino acid uptake studies in the Xenopus laevis
oocyte system.
GLAST is specific for L-glutamate and L-aspartate, shows strict dependence on Na+ ions, and is inhibited by DL-threo-3-hydroxy-aspartate. In situ hybridization reveals a strikingly high density of
GLAST mRNA in the Purkinje cell layer of
cerebellum, presumably in the Bergmann
glia cells, and a less dense distribution throughout the cerebrum. These data suggest that
GLAST may be involved in the regulation of neurotransmitter concentration in
central nervous system.
PubMed ID:
1279699
PMC ID:
PMC50461
Article link:
Proc Natl Acad Sci U S A
Species referenced:
Xenopus laevis
Genes referenced:
slc1a3
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