XB-ART-23292Nature October 8, 1992; 359 (6395): 500-5.
Mutations in the channel domain of a neuronal nicotinic receptor convert ion selectivity from cationic to anionic.
Introduction by site-directed mutagenesis of three amino acids from the MII segment of glycine or gamma-aminobutyric acid (GABAA) receptors into the MII segment of alpha 7 nicotinic receptor was sufficient to convert a cation-selective channel into an anion-selective channel gated by acetylcholine. A critical mutation was the insertion of an uncharged residue at the amino-terminal end of MII, stressing the importance of protein geometrical constraints on ion selectivity.
PubMed ID: 1383829
Article link: Nature
Genes referenced: gabarap