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XB-ART-2340
Development March 1, 2005; 132 (5): 1021-34.

BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation.

Tao J , Kuliyev E , Wang X , Li X , Wilanowski T , Jane SM , Mead PE , Cunningham JM .


Abstract
Morphogen-dependent epidermal-specific transacting factors have not been defined in vertebrates. We demonstrate that a member of the grainyhead transcription factor family, Grainyhead-like 1 (XGrhl1) is essential for ectodermal ontogeny in Xenopus laevis. Expression of this factor is restricted to epidermal cells. Moreover, XGrhl1 is regulated by the BMP4 signaling cascade. Disruption of XGrhl1 activity in vivo results in a severe defect in terminal epidermal differentiation, with inhibition of XK81A1 epidermal keratin gene expression, a key target of BMP4 signaling. Furthermore, transcription of the XK81A1 gene is modulated directly by binding of XGRHL1 to a promoter-localized binding motif that is essential for high-level expression. These results establish a novel developmental role for XGrhl1 as a crucial tissue-specific regulator of vertebrate epidermal differentiation.

PubMed ID: 15705857
Article link: Development

Genes referenced: bmp4 gmnn grhl1 krt12.4 lmo2 ncam1 nog nr3c1 odc1 smad1 tbx2 ventx2.1 ventx2.2
Morpholinos: grhl1 MO1


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