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Proc Natl Acad Sci U S A
2005 Feb 22;1028:2778-83. doi: 10.1073/pnas.0405374102.
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Nucleoplasmin regulates chromatin condensation during apoptosis.
Lu Z
,
Zhang C
,
Zhai Z
.
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Although chromatin condensation is one of the hallmarks of apoptosis, its relationship with DNA fragmentation has been controversial. We show here that apoptotic chromatin condensation is regulated by nucleoplasmin, a protein that decondenses sperm chromatin during male pronuclear assembly. In Xenopus egg extracts, nucleoplasmin is tyrosine-dephosphorylated during apoptosis. This dephosphorylation inactivates the chromatin decondensation activity of nucleoplasmin and leads to its exclusion from the chromatin. Inhibition of tyrosine dephosphorylation prevents apoptotic chromatin condensation but not DNA fragmentation. Studies with mutant proteins indicate that dephosphorylation of nucleoplasmin at Tyr-124 regulates chromatin condensation through changes in the interaction of nucleoplasmin with chromatin and the loss of its chromatin decondensation activity. These results show that chromatin condensation and DNA fragmentation are independent processes.
Asante-Appiah,
The YRD motif is a major determinant of substrate and inhibitor specificity in T-cell protein-tyrosine phosphatase.
2001, Pubmed
Asante-Appiah,
The YRD motif is a major determinant of substrate and inhibitor specificity in T-cell protein-tyrosine phosphatase.
2001,
Pubmed
Bezvenyuk,
Chromatin condensation during glutamate-induced excitotoxicity of cerebellar [correction of celebellar] granule neurons precedes disintegration of nuclear DNA into high molecular weight DNA fragments.
2003,
Pubmed
Budihardjo,
Biochemical pathways of caspase activation during apoptosis.
1999,
Pubmed
Colombo,
Nucleophosmin regulates the stability and transcriptional activity of p53.
2002,
Pubmed
Dalenc,
Increased expression of a COOH-truncated nucleophosmin resulting from alternative splicing is associated with cellular resistance to ionizing radiation in HeLa cells.
2002,
Pubmed
Earnshaw,
Assembly of nucleosomes: the reaction involving X. laevis nucleoplasmin.
1980,
Pubmed
,
Xenbase
Enari,
A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.
1998,
Pubmed
Ferrando-May,
Caspases mediate nucleoporin cleavage, but not early redistribution of nuclear transport factors and modulation of nuclear permeability in apoptosis.
2001,
Pubmed
Gupta,
A nuclear protein tyrosine phosphatase activates p53 and induces caspase-1-dependent apoptosis.
2002,
Pubmed
Hierro,
Structural and functional properties of Escherichia coli-derived nucleoplasmin. A comparative study of recombinant and natural proteins.
2001,
Pubmed
,
Xenbase
Lam,
Cellular stress regulates the nucleocytoplasmic distribution of the protein-tyrosine phosphatase TCPTP.
2001,
Pubmed
Leno,
Hyperphosphorylation of nucleoplasmin facilitates Xenopus sperm decondensation at fertilization.
1996,
Pubmed
,
Xenbase
Li,
Endonuclease G is an apoptotic DNase when released from mitochondria.
2001,
Pubmed
Liu,
The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis.
1998,
Pubmed
Lorenzen,
COOH-terminal sequence motifs target the T cell protein tyrosine phosphatase to the ER and nucleus.
1995,
Pubmed
Lu,
LDFF, the large molecular weight DNA fragmentation factor, is responsible for the large molecular weight DNA degradation during apoptosis in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase
Marsden,
Control of apoptosis in the immune system: Bcl-2, BH3-only proteins and more.
2003,
Pubmed
Nambirajan,
PTP-S2, a nuclear tyrosine phosphatase, is phosphorylated and excluded from condensed chromosomes during mitosis.
2000,
Pubmed
Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase
Philpott,
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Radha,
Induction of p53 dependent apoptosis upon overexpression of a nuclear protein tyrosine phosphatase.
1999,
Pubmed
Sakahira,
Apoptotic nuclear morphological change without DNA fragmentation.
1999,
Pubmed
Susin,
Molecular characterization of mitochondrial apoptosis-inducing factor.
1999,
Pubmed
