Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1992 Aug 01;118:2903-8. doi: 10.1002/j.1460-2075.1992.tb05359.x.
Show Gene links
Show Anatomy links
Xenopus MAP kinase activator is a serine/threonine/tyrosine kinase activated by threonine phosphorylation.
Kosako H
,
Gotoh Y
,
Matsuda S
,
Ishikawa M
,
Nishida E
.
???displayArticle.abstract???
Xenopus MAP kinase activator, a 45 kDa protein, has been shown to function as a direct upstream factor sufficient for full activation and both tyrosine and serine/threonine phosphorylation of inactive MAP kinase. We have now shown by using an anti-MAP kinase activator antiserum that MAP kinase activator is ubiquitous in tissues and is regulated post-translationally. Activation of MAP kinase activator is correlated precisely with its threonine phosphorylation during the oocyte maturation process. It is a key question whether MAP kinase activator is a kinase or not. We have shown that Xenopus MAP kinase activator purified from mature oocytes is capable of undergoing autophosphorylation on serine, threonine and tyrosine residues. Dephosphorylation of purified activator by protein phosphatase 2A treatment inactivates its autophosphorylation activity as well as its activator activity. Thus, Xenopus MAP kinase activator is a protein kinase with specificity for both serine/threonine and tyrosine. Partial protein sequencing of purified activator indicates that it contains a sequence homologous to kinase subdomains VI and VII of two yeast protein kinases, STE7 and byrl.
Ahn,
Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase.
1991, Pubmed
Ahn,
Multiple components in an epidermal growth factor-stimulated protein kinase cascade. In vitro activation of a myelin basic protein/microtubule-associated protein 2 kinase.
1991,
Pubmed
Ahn,
Identification of multiple epidermal growth factor-stimulated protein serine/threonine kinases from Swiss 3T3 cells.
1990,
Pubmed
Alvarez,
Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase.
1991,
Pubmed
Anderson,
Requirement for integration of signals from two distinct phosphorylation pathways for activation of MAP kinase.
1990,
Pubmed
Boulton,
ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.
1991,
Pubmed
Boulton,
An insulin-stimulated protein kinase similar to yeast kinases involved in cell cycle control.
1990,
Pubmed
Boyle,
Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates.
1991,
Pubmed
Chung,
Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity.
1991,
Pubmed
Courchesne,
A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae.
1989,
Pubmed
Crews,
Mouse Erk-1 gene product is a serine/threonine protein kinase that has the potential to phosphorylate tyrosine.
1991,
Pubmed
Elion,
FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation.
1990,
Pubmed
Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase
Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase
Gotoh,
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF.
1991,
Pubmed
,
Xenbase
Gotoh,
Microtubule-associated-protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogen-activated MAP kinase of fibroblastic cells.
1990,
Pubmed
Gotoh,
Okadaic acid activates microtubule-associated protein kinase in quiescent fibroblastic cells.
1990,
Pubmed
Gómez,
Dissection of the protein kinase cascade by which nerve growth factor activates MAP kinases.
1991,
Pubmed
Gómez,
Evidence for communication between nerve growth factor and protein tyrosine phosphorylation.
1990,
Pubmed
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Hoshi,
Characterization of a mitogen-activated, Ca2+-sensitive microtubule-associated protein-2 kinase.
1989,
Pubmed
Hoshi,
Activation of a Ca2+-inhibitable protein kinase that phosphorylates microtubule-associated protein 2 in vitro by growth factors, phorbol esters, and serum in quiescent cultured human fibroblasts.
1988,
Pubmed
Matsuda,
Xenopus MAP kinase activator: identification and function as a key intermediate in the phosphorylation cascade.
1992,
Pubmed
,
Xenbase
Miyasaka,
Nerve growth factor stimulates a protein kinase in PC-12 cells that phosphorylates microtubule-associated protein-2.
1990,
Pubmed
Nadin-Davis,
Schizosaccharomyces pombe ras1 and byr1 are functionally related genes of the ste family that affect starvation-induced transcription of mating-type genes.
1990,
Pubmed
Nadin-Davis,
A gene which encodes a predicted protein kinase can restore some functions of the ras gene in fission yeast.
1988,
Pubmed
Pelech,
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization.
1988,
Pubmed
Posada,
Requirements for phosphorylation of MAP kinase during meiosis in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Posada,
Tyrosine phosphorylation and activation of homologous protein kinases during oocyte maturation and mitogenic activation of fibroblasts.
1991,
Pubmed
,
Xenbase
Pulverer,
Phosphorylation of c-jun mediated by MAP kinases.
1991,
Pubmed
Ray,
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro.
1987,
Pubmed
Ray,
Insulin-stimulated microtubule-associated protein kinase is phosphorylated on tyrosine and threonine in vivo.
1988,
Pubmed
Sanghera,
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
1990,
Pubmed
Seger,
Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: implications for their mechanism of activation.
1991,
Pubmed
Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase
Teague,
Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.
1986,
Pubmed
Toda,
Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.
1991,
Pubmed
Wu,
Autophosphorylation in vitro of recombinant 42-kilodalton mitogen-activated protein kinase on tyrosine.
1991,
Pubmed
