Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA.
Beltrame M
,
Tollervey D
.
???displayArticle.abstract???
It has long been known that U3 can be isolated hydrogen bonded to pre-ribosomal RNAs, but the sites of interaction are poorly characterized. Here we show that yeast U3 can be cross-linked to 35S pre-rRNA both in deproteinized extracts and in living cells. The sites of cross-linking were localized to the 5' external transcribed spacer (ETS) and then identified at the nucleotide level. Two regions of U3 near the 5' end are cross-linked to pre-rRNA in vivo and in vitro; the evolutionarily conserved box A region and a 10 nucleotide (nt) sequence with perfect complementarity to an ETS sequence. Two in vivo cross-links are detected in the ETS, at +470, within the region complementary to U3, and at +655, close to the cleavage site at the 5' end of 18S rRNA. A tagged rDNA construct was used to follow the effects of mutations in the ETS in vivo. A small deletion around the +470 cross-linking site in the ETS prevents the synthesis of 18S rRNA. This region is homologous to the site of vertebrate ETS cleavage. We propose that this site may be evolutionarily conserved to direct the assembly of a pre-rRNA processing complex required for the cleavages that generate 18S rRNA.
Bachellerie,
Recognition signals for mouse pre-rRNA processing. A potential role for U3 nucleolar RNA.
1983, Pubmed
Bachellerie,
Recognition signals for mouse pre-rRNA processing. A potential role for U3 nucleolar RNA.
1983,
Pubmed
Boorstein,
Primer extension analysis of RNA.
1989,
Pubmed
Clark,
SSB-1 of the yeast Saccharomyces cerevisiae is a nucleolar-specific, silver-binding protein that is associated with the snR10 and snR11 small nuclear RNAs.
1990,
Pubmed
Crouch,
A model for the involvement of the small nucleolar RNA (U3) in processing eukaryotic ribosomal RNA.
1983,
Pubmed
Girard,
GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast.
1992,
Pubmed
Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed
Hughes,
The yeast homologue of U3 snRNA.
1987,
Pubmed
,
Xenbase
Ito,
Transformation of intact yeast cells treated with alkali cations.
1983,
Pubmed
Jansen,
Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast.
1991,
Pubmed
,
Xenbase
Kass,
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
1990,
Pubmed
Kass,
The first pre-rRNA-processing event occurs in a large complex: analysis by gel retardation, sedimentation, and UV cross-linking.
1990,
Pubmed
Kass,
Primary processing of mammalian rRNA involves two adjacent cleavages and is not species specific.
1987,
Pubmed
Klootwijk,
Isolation and characterization of yeast ribosomal RNA precursors and preribosomes.
1989,
Pubmed
Li,
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
1990,
Pubmed
Maden,
Nucleotide sequence of an external transcribed spacer in Xenopus laevis rDNA: sequences flanking the 5' and 3' ends of 18S rRNA are non-complementary.
1982,
Pubmed
,
Xenbase
Maser,
U3 small nuclear RNA can be psoralen-cross-linked in vivo to the 5' external transcribed spacer of pre-ribosomal-RNA.
1989,
Pubmed
Michot,
Secondary structure of the 5' external transcribed spacer of vertebrate pre-rRNA. Presence of phylogenetically conserved features.
1991,
Pubmed
,
Xenbase
Musters,
Functional analysis of transcribed spacers of yeast ribosomal DNA.
1990,
Pubmed
Musters,
A system for the analysis of yeast ribosomal DNA mutations.
1989,
Pubmed
Myslinski,
An intron in the genes for U3 small nucleolar RNAs of the yeast Saccharomyces cerevisiae.
1990,
Pubmed
Parker,
Structural analysis of the human U3 ribonucleoprotein particle reveal a conserved sequence available for base pairing with pre-rRNA.
1987,
Pubmed
Parker,
An in vitro interaction between the human U3 snRNP and 28S rRNA sequences near the alpha-sarcin site.
1988,
Pubmed
Porter,
The sequence of U3 from Schizosaccharomyces pombe suggests structural divergence of this snRNA between metazoans and unicellular eukaryotes.
1988,
Pubmed
Prestayko,
Low molecular weight RNA associated with 28 s nucleolar RNA.
1970,
Pubmed
Savino,
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
1990,
Pubmed
,
Xenbase
Schimmang,
A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability.
1989,
Pubmed
Stroke,
The 5' end of U3 snRNA can be crosslinked in vivo to the external transcribed spacer of rat ribosomal RNA precursors.
1989,
Pubmed
Tague,
Processing of the large rRNA precursor: two proposed categories of RNA-RNA interactions in eukaryotes.
1984,
Pubmed
Tollervey,
The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.
1991,
Pubmed
Tollervey,
The role of small nucleolar ribonucleoproteins in ribosome synthesis.
1990,
Pubmed
Tollervey,
Deletion of a yeast small nuclear RNA gene impairs growth.
1985,
Pubmed
Tollervey,
Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs.
1987,
Pubmed
,
Xenbase
Tollervey,
A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.
1987,
Pubmed
Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed
Zagorski,
Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs.
1988,
Pubmed
