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J Cell Biol January 31, 2005; 168 (3): 429-39.

Concentric zones of active RhoA and Cdc42 around single cell wounds.

Benink HA , Bement WM .

Rho GTPases control many cytoskeleton-dependent processes, but how they regulate spatially distinct features of cytoskeletal function within a single cell is poorly understood. Here, we studied active RhoA and Cdc42 in wounded Xenopus oocytes, which assemble and close a dynamic ring of actin filaments (F-actin) and myosin-2 around wound sites. RhoA and Cdc42 are rapidly activated around wound sites in a calcium-dependent manner and segregate into distinct, concentric zones around the wound, with active Cdc42 in the approximate middle of the F-actin array and active RhoA on the interior of the array. These zones form before F-actin accumulation, and then move in concert with the closing array. Microtubules and F-actin are required for normal zone organization and dynamics, as is crosstalk between RhoA and Cdc42. Each of the zones makes distinct contributions to the organization and function of the actomyosin wound array. We propose that similar rho activity zones control related processes such as cytokinesis.

PubMed ID: 15684032
PMC ID: PMC2171735
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: actl6a cdc42 mlc1 noct rho rho.2 rhoa rhoa.2

Article Images: [+] show captions
References [+] :
Aktories, Rho-modifying C3-like ADP-ribosyltransferases. 2004, Pubmed