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Metabolic changes with fatigue in different types of single muscle fibres of Xenopus laevis.
Nagesser AS
,
van der Laarse WJ
,
Elzinga G
.
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1. Peak isometric force of single fast (type 1) and slow (type 3) muscle fibres of Xenopus decreased when fibres were stimulated intermittently above their predicted sustainable duty cycle at 20 degrees C. Type 1 fibres could be fatigued to zero force. In most type 3 fibres force did not decrease below 50% of the original (P0) before activation failure, as indicated by irregular contractions. 2. Fibres were rapidly frozen at different force levels and analysed by high-performance liquid chromatography (HPLC) for ATP, IMP, phosphocreatine (PCr) and creatine (Cr). Lactate was determined enzymatically in type 1 fibres only. The relationships between force and PCr, and between force and ATP during fatigue were, apart from the range of values obtained, the same for both fibre types. When force had fallen to about 60-80% of original, PCr was fully reduced. At lower force levels, the ATP content-decreased, and a concomitant rise of IMP content was found. At zero force, ATP had fallen to about 25% of its value in rested type 1 fibres, and up to 200 mumol lactate (g dry weight)-1 had accumulated. 3. Recovery from fatigue was studied in fibres where force had fallen to 0.6 P0 (both fibre types) and 0.2 P0 (type 1 only). After 1 h of recovery ATP had in all cases returned to the level measured in rested fibres. In fibres fatigued to 0.6 P0, force almost returned to its original value. However, in type 1 fibres fatigued to 0.2 P0, it returned to only 0.3 P0. After 1 h of recovery the PCr/Cr ratio in type 1 fibres was lower (probability, P less than 0.05) than in control fibres, whereas in type 3 fibres it was not significantly different from controls. 4. The relationship between peak force and stimulus frequency, which had a sigmoid shape in fully rested fibres, was drastically changed by fatiguing stimulation. Immediately after fatiguing stimulation of type 1 fibres, force hardly increased with stimulus frequency, corresponding to the observation that calcium efflux from the sarcoplasmic reticulum was decreased at high stimulus frequencies. The force-frequency relationship of type 3 fibres was the same before and after intermittent stimulation.
Allen,
Intracellular calcium and tension during fatigue in isolated single muscle fibres from Xenopus laevis.
1989, Pubmed,
Xenbase
Allen,
Intracellular calcium and tension during fatigue in isolated single muscle fibres from Xenopus laevis.
1989,
Pubmed
,
Xenbase
Burke,
Physiological types and histochemical profiles in motor units of the cat gastrocnemius.
1973,
Pubmed
Chase,
Effects of pH on contraction of rabbit fast and slow skeletal muscle fibers.
1988,
Pubmed
Cooke,
The inhibition of rabbit skeletal muscle contraction by hydrogen ions and phosphate.
1988,
Pubmed
Crow,
Chemical energetics of slow- and fast-twitch muscles of the mouse.
1982,
Pubmed
Curtin,
Energy changes and muscular contraction.
1978,
Pubmed
Dawson,
Muscular fatigue investigated by phosphorus nuclear magnetic resonance.
1978,
Pubmed
Edman,
Effects of fatigue and altered pH on isometric force and velocity of shortening at zero load in frog muscle fibres.
1981,
Pubmed
Edman,
The force-velocity relationship in vertebrate muscle fibres at varied tonicity of the extracellular medium.
1977,
Pubmed
Edwards,
Metabolic changes associated with the slowing of relaxation in fatigued mouse muscle.
1975,
Pubmed
Elzinga,
Stable maintenance heat rate and contractile properties of different single muscle fibres from Xenopus laevis at 20 degrees C.
1987,
Pubmed
,
Xenbase
Fabiato,
Effects of pH on the myofilaments and the sarcoplasmic reticulum of skinned cells from cardiace and skeletal muscles.
1978,
Pubmed
Godt,
Changes of intracellular milieu with fatigue or hypoxia depress contraction of skinned rabbit skeletal and cardiac muscle.
1989,
Pubmed
Gonzalez-Serratos,
Composition of vacuoles and sarcoplasmic reticulum in fatigued muscle: electron probe analysis.
1978,
Pubmed
Gunter,
Mechanisms by which mitochondria transport calcium.
1990,
Pubmed
Juengling,
Rapid assay of adenine nucleotides or creatine compounds in extracts of cardiac tissue by paired-ion reverse-phase high-performance liquid chromatography.
1980,
Pubmed
KREBS,
Renal gluconeogenesis. The effect of diet on the gluconeogenic capacity of rat-kidney-cortex slices.
1963,
Pubmed
Lamb,
Effect of Mg2+ on the control of Ca2+ release in skeletal muscle fibres of the toad.
1991,
Pubmed
Lännergren,
Transient appearance of vacuoles in fatigued Xenopus muscle fibres.
1990,
Pubmed
,
Xenbase
Lännergren,
Maximum tension and force-velocity properties of fatigued, single Xenopus muscle fibres studied by caffeine and high K+.
1989,
Pubmed
,
Xenbase
Nassar-Gentina,
Fatigue and metabolism of frog muscle fibers during stimulation and in response to caffeine.
1981,
Pubmed
Nassar-Gentina,
Metabolic correlates of fatigue and of recovery from fatigue in single frog muscle fibers.
1978,
Pubmed
Smith,
Varieties of fast and slow extrafusal muscle fibres in amphibian hind limb muscles.
1973,
Pubmed
,
Xenbase
Spurway,
Quantitative analysis of histochemical and immunohistochemical reactions in skeletal muscle fibres of Rana and Xenopus.
1989,
Pubmed
,
Xenbase
Taylor,
Energetics of human muscle: exercise-induced ATP depletion.
1986,
Pubmed
Westerblad,
The relation between force and intracellular pH in fatigued, single Xenopus muscle fibres.
1988,
Pubmed
,
Xenbase
Wilkie,
Muscular fatigue: effects of hydrogen ions and inorganic phosphate.
1986,
Pubmed
van der Laarse,
Resistance to fatigue of single muscle fibres from Xenopus related to succinate dehydrogenase and myofibrillar ATPase activities.
1991,
Pubmed
,
Xenbase
van der Laarse,
The duty cycle of single muscle fibers from Xenopus laevis.
1989,
Pubmed
,
Xenbase
van der Laarse,
Maximum rate of oxygen consumption and quantitative histochemistry of succinate dehydrogenase in single muscle fibres of Xenopus laevis.
1989,
Pubmed
,
Xenbase
