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XB-ART-2399
Dev Biol February 15, 2005; 278 (2): 542-59.

The ARID domain protein dril1 is necessary for TGF(beta) signaling in Xenopus embryos.



Abstract
ARID domain proteins are members of a highly conserved family involved in chromatin remodeling and cell-fate determination. Dril1 is the founding member of the ARID family and is involved in developmental processes in both Drosophila and Caenorhabditis elegans. We describe the first embryological characterization of this gene in chordates. Dril1 mRNA expression is spatiotemporally regulated and is detected in the involuting mesoderm during gastrulation. Inhibition of dril1 by either a morpholino or an engrailed repressor-dril1 DNA binding domain fusion construct inhibits gastrulation and perturbs induction of the zygotic mesodermal marker Xbra and the organizer markers chordin, noggin, and Xlim1. Xenopus tropicalis dril1 morphants also exhibit impaired gastrulation and axial deficiencies, which can be rescued by coinjection of Xenopus laevis dril1 mRNA. Loss of dril1 inhibits the response of animal caps to activin and secondary axis induction by smad2. Dril1 depletion in animal caps prevents both the smad2-mediated induction of dorsal mesodermal and endodermal markers and the induction of ventral mesoderm by smad1. Mesoderm induction by eFGF is uninhibited in dril1 morphant caps, reflecting pathway specificity for dril1. These experiments identify dril1 as a novel regulator of TGF(beta) signaling and a vital component of mesodermal patterning and embryonic morphogenesis.

PubMed ID: 15680369
Article link: Dev Biol
Grant support: [+]
Genes referenced: arid3a bambi chrd.1 clstn2 eomes fgf4 foxa4 frzb fzd7 gsc h3-3a hnf1a lhx1 mix1 myc nog prickle1 smad1 smad2 smad7 spry2 tbxt ventx2.2
Antibodies: H3f3a Ab9
Morpholinos: arid3a MO1


Article Images: [+] show captions


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