Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Protein synthesis in the newt regenerating limb. Comparative two-dimensional PAGE, computer analysis and protein sequencing.
Tsonis PA
,
Mescher AL
,
Del Rio-Tsonis K
.
???displayArticle.abstract???
Protein synthesis has been studied by two-dimensional PAGE during the early limb regeneration in the adult newt. Quantitative and statistical analyses have provided unique information on overall patterns of protein synthesis as well as on specific protein synthesis during formation of the blastema. Furthermore, from the patterns in the two-dimensional gels and their quantification, a particular protein has been selected and sequenced. Partial sequencing revealed sequence similarities to Xenopus type I keratin B2. Expression of this keratin is 10-fold greater in the blastema than in the intact limb. The implications of keratin expression by the blastema cells are discussed.
Brockes,
Retinoids, homeobox genes, and limb morphogenesis.
1989, Pubmed
Brockes,
Retinoids, homeobox genes, and limb morphogenesis.
1989,
Pubmed
Donaldson,
Protein patterns during regeneration and wound healing in the adult newt: a comparative study using gel electrophoresis.
1974,
Pubmed
Ferretti,
Culture of newt cells from different tissues and their expression of a regeneration-associated antigen.
1988,
Pubmed
Ferretti,
A newt type II keratin restricted to normal and regenerating limbs and tails is responsive to retinoic acid.
1991,
Pubmed
Ferretti,
Transient expression of simple epithelial keratins by mesenchymal cells of regenerating newt limb.
1989,
Pubmed
Garrels,
Quantitative two-dimensional gel electrophoresis of proteins.
1983,
Pubmed
Garrels,
The QUEST system for quantitative analysis of two-dimensional gels.
1989,
Pubmed
Giguère,
Spatial and temporal expression of the retinoic acid receptor in the regenerating amphibian limb.
1989,
Pubmed
Maden,
Vitamin A and pattern formation in the regenerating limb.
1982,
Pubmed
Miyatani,
Stage-specific keratins in Xenopus laevis embryos and tadpoles: the XK81 gene family.
1986,
Pubmed
,
Xenbase
Ragsdale,
Identification of a novel retinoic acid receptor in regenerative tissues of the newt.
1989,
Pubmed
Savard,
Position dependent expression of a homeobox gene transcript in relation to amphibian limb regeneration.
1988,
Pubmed
,
Xenbase
Slack,
Protein synthesis during limb regeneration in the axolotl.
1982,
Pubmed
Tabin,
Isolation of potential vertebrate limb-identity genes.
1989,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Tsonis,
Molecular approaches in limb development and regeneration.
1990,
Pubmed
