XB-ART-2423J Biol Chem. April 8, 2005; 280 (14): 14122-9.
Bone morphogenetic proteins (BMPs) are members of the transforming growth factor (TGF)beta superfamily of ligands that regulate many crucial aspects of embryonic development and organogenesis. Unlike other TGFbeta ligands, co-receptors for BMP ligands have not been described. Here we show that DRAGON, a glycosylphosphatidylinositol-anchored member of the repulsive guidance molecule family, which is expressed early in the developing nervous system, enhances BMP but not TGFbeta signaling. DRAGON binds directly to BMP2 and BMP4 but not to BMP7 or other TGFbeta ligands. The enhancing action of DRAGON on BMP signaling is also reduced by administration of Noggin, a soluble BMP antagonist, indicating that the action of DRAGON is ligand-dependent. DRAGON associates directly with BMP type I (ALK2, ALK3, and ALK6) and type II (ActRII and ActRIIB) receptors, and its signaling is reduced by dominant negative Smad1 and ALK3 or -6 receptors. In the Xenopus embryo, DRAGON both reduces the threshold of the ability of Smad1 to induce mesodermal and endodermal markers and alters neuronal and neural crest patterning. The direct interaction of DRAGON with BMP ligands and receptors indicates that it is a BMP co-receptor that potentiates BMP signaling.
PubMed ID: 15671031
Article link: J Biol Chem.
Genes referenced: acta1 acvr1 acvr2a acvr2b acvrl1 alk bmp2 bmp4 bmp7.1 bmp7.2 bmpr1a bmpr1b bre csrp3 gnpda1 inhba nkx2-5 nog nr2e1 nrp1 otx2 pklr rgma rgmb smad1 sox2 t tgfb1 tubb2b twist1
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|FIG. 1. DRAGON is expressed during embryogenesis. A, DRAGON expression in mouse pre-implantation embryos (E2.5) assessed with a DRAGON antibody and visualized by fluorescein isothiocyanate green fluorescence. Nuclei of the embryonic cells are visualized by CY3 red fluorescence (propidium iodide). Pre-adsorption of the DRAGON antibody with the peptide antigen (Pept.) was used as negative control. B, Northern blot showing DRAGON mRNA expression in mouse embryos at embryonic stages E7–17. Cyclophilin mRNA levels were used as loading controls. C, immunohistochemical study of the expression and distribution of DRAGON in mouse E10.5 embryos. D, expression of Xenopus DRAGON (RGMb) and RGMa assessed by reverse transcription (RT)-PCR at the indicated developmental stages. Orthidine decarboxylase (ODC) levels were used as internal control. E, analysis by whole mount in situ hybridization of expression of Xenopus RGMa at stages 12 (i), 17 (ii), 20 (iii), and 35 (iv). Transverse sections through stained stage 12 (top right) and stage 20 (bottom left) embryos are also represented. bp, blastopore; post, posterior; ant, anterior; nc, notochord; rp, roof plate; hb, hindbrain; mb, midbrain; fb, forebrain; ba, branchial arches.|
|FIG. 4. DRAGON-mediated BMP signaling requires its GPI anchor. A, DRAGON-Fc (DR-Fc) when expressed by LLC-PK1 cells does not increase BMP signaling. B, Expression of both full-length DRAGON (DR) and DRAGON-Fc by the LLC-PK1 cells was confirmed by Western blot analysis.|
|FIG. 6. DRAGON potentiates BMP signaling in Xenopus embryos. A, microinjection of mouse DRAGON mRNA in Xenopus laevis embryos at gastrula stages synergizes with Smad1 (mRNA injection) to induce mesodermal (xBra and mix1) and endodermal (mixer) markers, as assessed by reverse transcription (RT)- PCR. No induction of EK or Sox2 occurs. B, in animal cap explants DRAGON mRNA injection induces expression of the anterior neural markers nrp1 and Otx2, as well the as cement gland marker (Xag) and nkx2.5, a heart marker. Orthidine decarboxylase (ODC) is the loading control. C, Xenopus embryos were injected in the animal pole of one of two cells at the two-cell stage with DRAGON mRNA and analyzed at late neurula (stage 23), for changes in neural crest patterning, and early tadpole stages (stage 28) for ectopic induction of neural tissue. DRAGON overexpression inhibits twist RNA, a neural crest marker (top panels), and induces ectopic patches of N-tubulin, a neural marker (lower panels).|
|rgmb (repulsive guidance molecule family member b) gene expression in bissected Xenopus laevis embryo, assayed via in situ hybridization, NF stage 12, lateral view, dorsal right.|
|rgmb (repulsive guidance molecule family member b) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 17, anterior view, dorsal left|
|rgmb (repulsive guidance molecule family member b) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 35, lateral view, anterior right, dorsal up.|