XB-ART-24586
EMBO J
September 1, 1991;
10
(9):
2653-9.
A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.
Elledge SJ
,
Spottswood MR
.
Abstract
The onset of S-phase and M-phase in both Schizosaccharomyces pombe and Saccharomyces cerevisiae requires the function of the
cdc2/CDC28 gene product,
p34, a serine-threonine protein kinase. A human homolog,
p34cdc2, was identified by functional complementation of the S.pombe
cdc2 mutation (Lee and Nurse, 1987). Using a human cDNA expression library to search for suppressors of cdc28 mutations in S. cerevisiae, we have identified a second functional
p34 homolog,
CDK2 cell division kinase). This gene is expressed as a 2.1 kb transcript encoding a polypeptide of 298 amino acids. This protein retains nearly all of the amino acids highly conserved among previously identified
p34 homologs from other species, but is considerably divergent from all previous
p34cdc2 homologs, approximately 65% identity. This gene encodes the human homolog of the Xenopus
Eg1 gene, sharing 89% amino acid identity, and defines a second sub-family of
CDC2 homologs. A second chromosomal mutation which arose spontaneously was required to allow complementation of the cdc28-4 mutation by
CDK2. This mutation blocked the ability of this strain to mate. These results suggest that the machinery controlling the human cell cycle is more complex than that for fission and budding yeast.
PubMed ID:
1714386
PMC ID:
PMC452966
Article link:
Grant support:
[+]
Species referenced:
Xenopus laevis
Genes referenced:
aagab
cdk1
cdk2
pold1
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