XB-ART-24651Proc Natl Acad Sci U S A August 1, 1991; 88 (15): 6511-4.
Presence of activin (erythroid differentiation factor) in unfertilized eggs and blastulae of Xenopus laevis.
Activin A, a member of the transforming growth factor beta superfamily, has recently been found to have potent mesoderm-inducing activity on isolated early Xenopus animal-cap cells. We measured the activin activity of the Xenopus egg extract by using an erythroid-differentiating test with Friend leukemia cells. The results showed that an activin homologue is, indeed, contained in unfertilized eggs and blastulae of Xenopus laevis in a considerable amount. This activity was eluted at the same retention time as human activin A when fractionated by reversed-phase HPLC. Furthermore, the fraction containing erythroid-differentiating factor activity had mesoderm-inducing activity on Xenopus animal-cap cells. The mesoderm-inducing activity of this fraction was suppressed when coincubated with follistatin, an activin-binding protein. These results suggest that an endogenous activin may be a natural mesoderm-inducing factor acting in Xenopus embryogenesis.
PubMed ID: 1862079
PMC ID: PMC52115
Article link: Proc Natl Acad Sci U S A
Species referenced: Xenopus laevis
Genes referenced: fst inhba
References [+] :
Asashima, The vegetalizing factor belongs to a family of mesoderm-inducing proteins related to erythroid differentiation factor. 1991, Pubmed, Xenbase