Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1991 May 01;115:2656-64. doi: 10.1128/mcb.11.5.2656-2664.1991.
Show Gene links
Show Anatomy links
Both the 5' untranslated region and the sequences surrounding the start site contribute to efficient initiation of translation in vitro.
Falcone D
,
Andrews DW
.
???displayArticle.abstract???
The role of RNA sequences in the 5' leader region between the cap site and initiating AUG in mediating translation was examined in vitro. Hybrid mRNAs were synthesized in which the cognate leader sequence was replaced with either optimized or compromised leader sequences, and translational efficiency was measured for six different coding regions. Translation was most efficient with a leader containing the 5' untranslated region from Xenopus beta-globin and an optimized initiation sequence. Compared with the cognate leaders, this hybrid was observed to increase translation of the various coding regions as much as 300-fold. The translational efficiencies of the different coding regions also varied substantially. In contrast to earlier suggestions that increased leader efficiency results from higher affinity of the leader for a limiting factor, our experiments suggest that increased translation from the beta-globin hybrid leader sequence results from more rapid initiation of translation.
Andrews,
Sequences beyond the cleavage site influence signal peptide function.
1988, Pubmed
Andrews,
Sequences beyond the cleavage site influence signal peptide function.
1988,
Pubmed
Andrews,
Examining protein translocation in cell-free systems and microinjected Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Banerjee,
5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
1980,
Pubmed
Berkner,
Abundant expression of polyomavirus middle T antigen and dihydrofolate reductase in an adenovirus recombinant.
1987,
Pubmed
Brown,
Requirements for the translational repression of ferritin transcripts in wheat germ extracts by a 90-kDa protein from rabbit liver.
1989,
Pubmed
Cigan,
Sequence and structural features associated with translational initiator regions in yeast--a review.
1987,
Pubmed
Daniels-McQueen,
Identification of mRNAs encoding the alpha and beta subunits of human choriogonadotropin.
1978,
Pubmed
Dasso,
On the fidelity of mRNA translation in the nuclease-treated rabbit reticulocyte lysate system.
1989,
Pubmed
Elroy-Stein,
Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.
1989,
Pubmed
Gallie,
The 5'-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo.
1987,
Pubmed
,
Xenbase
Gallie,
A comparison of eukaryotic viral 5'-leader sequences as enhancers of mRNA expression in vivo.
1987,
Pubmed
,
Xenbase
Gallie,
Mutational analysis of the tobacco mosaic virus 5'-leader for altered ability to enhance translation.
1988,
Pubmed
,
Xenbase
Gehrke,
5'-Conformation of capped alfalfa mosaic virus ribonucleic acid 4 may reflect its independence of the cap structure or of cap-binding protein for efficient translation.
1983,
Pubmed
Gerstenfeld,
Cell-free translation of calf type III collagen. Effect of magnesium on ribosome movement during elongation.
1983,
Pubmed
Jackson,
Do the poly(A) tail and 3' untranslated region control mRNA translation?
1990,
Pubmed
Jobling,
Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence.
,
Pubmed
Jobling,
In vitro transcription and translational efficiency of chimeric SP6 messenger RNAs devoid of 5' vector nucleotides.
1988,
Pubmed
Johansen,
Affecting gene expression by altering the length and sequence of the 5' leader.
1984,
Pubmed
Klausner,
cis-trans models for post-transcriptional gene regulation.
1989,
Pubmed
Klemenz,
Selective translation of heat shock mRNA in Drosophila melanogaster depends on sequence information in the leader.
1985,
Pubmed
Konarska,
Binding of ribosomes to linear and circular forms of the 5'-terminal leader fragment of tobacco-mosaic-virus RNA.
1981,
Pubmed
Kozak,
How do eucaryotic ribosomes select initiation regions in messenger RNA?
1978,
Pubmed
Kozak,
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
1986,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kozak,
A profusion of controls.
1988,
Pubmed
Kozak,
Leader length and secondary structure modulate mRNA function under conditions of stress.
1988,
Pubmed
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Kozak,
Context effects and inefficient initiation at non-AUG codons in eucaryotic cell-free translation systems.
1989,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lightfoot,
Magnesium-dependence of in vitro translation programmed by gene-specific mRNAs.
1988,
Pubmed
Lingelbach,
An extended RNA/RNA duplex structure within the coding region of mRNA does not block translational elongation.
1988,
Pubmed
Lodish,
Translational control of protein synthesis.
1976,
Pubmed
Lütcke,
Selection of AUG initiation codons differs in plants and animals.
1987,
Pubmed
McGarry,
The preferential translation of Drosophila hsp70 mRNA requires sequences in the untranslated leader.
1985,
Pubmed
Morlé,
alpha-Thalassaemia associated with the deletion of two nucleotides at position -2 and -3 preceding the AUG codon.
1985,
Pubmed
Perara,
A former amino terminal signal sequence engineered to an internal location directs translocation of both flanking protein domains.
1985,
Pubmed
Ray,
ATP-dependent unwinding of messenger RNA structure by eukaryotic initiation factors.
1985,
Pubmed
Schägger,
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
1987,
Pubmed
Sonenberg,
ATP/Mg++-dependent cross-linking of cap binding proteins to the 5' end of eukaryotic mRNA.
1981,
Pubmed
Tahara,
Two forms of purified m7G-cap binding protein with different effects on capped mRNA translation in extracts of uninfected and poliovirus-infected HeLa cells.
1981,
Pubmed
Walden,
Purification of a specific repressor of ferritin mRNA translation from rabbit liver.
1989,
Pubmed
Weyer,
Functional analysis of the p10 gene 5' leader sequence of the Autographa californica nuclear polyhedrosis virus.
1988,
Pubmed
