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Dev Comp Immunol January 1, 2007; 31 (4): 383-93.

Cells of cutaneous immunity in Xenopus: studies during larval development and limb regeneration.

Mescher AL , Wolf WL , Moseman EA , Hartman B , Harrison C , Nguyen E , Neff AW .

The anuran Xenopus laevis is an experimental model for vertebrate development, immunology, and regenerative biology. Using histochemistry and immunohistochemistry (IHC) we examined embryonic, larval, and postmetamorphic Xenopus skin for the presence of dendritic cells (DCs), Langerhans cells (LCs), and dendritic epidermal T cells (DETCs), all components of cutaneous immunity that have been implicated in skin repair and regeneration. Cells expressing three markers for dendritic and Langerhans cells (formalin-resistant ATPase activity, major histocompatibility complex [MHC] class II antigens, and vimentin) and having morphology like that of these cells first appeared during late embryonic stages, becoming abundant by prometamorphosis. Cells positive for these markers were also numerous in the wound epithelia of regenerating hindlimbs at both early and late larval stages. Cells tentatively identified as DETCs were found, beginning at early larval stages, using IHC with antibodies against heterologous CD3epsilon chain and T-cell receptor delta. Further characterization and work with the putative DCs, LCs, and DETCs demonstrated here will allow not only greater understanding of the amphibian immune system, but also further elucidation of regenerative growth and scarring.

PubMed ID: 16926047
Article link: Dev Comp Immunol

Genes referenced: cd3e mhc2a tra vim
Antibodies: Mhc2a Ab1 Vim Ab1 cd3e Ab2

Article Images: [+] show captions

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