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XB-ART-2506
Development. February 1, 2005; 132 (3): 553-63.

Tbx5 and Tbx20 act synergistically to control vertebrate heart morphogenesis.

Brown DD , Martz SN , Binder O , Goetz SC , Price BM , Smith JC , Conlon FL .


Abstract
Members of the T-box family of proteins play a fundamental role in patterning the developing vertebrate heart; however, the precise cellular requirements for any one family member and the mechanism by which individual T-box genes function remains largely unknown. In this study, we have investigated the cellular and molecular relationship between two T-box genes, Tbx5 and Tbx20. We demonstrate that blocking Tbx5 or Tbx20 produces phenotypes that display a high degree of similarity, as judged by overall gross morphology, molecular marker analysis and cardiac physiology, implying that the two genes are required for and have non-redundant functions in early heart development. In addition, we demonstrate that although co-expressed, Tbx5 and Tbx20 are not dependent on the expression of one another, but rather have a synergistic role during early heart development. Consistent with this proposal, we show that TBX5 and TBX20 can physically interact and map the interaction domains, and we show a cellular interaction for the two proteins in cardiac development, thus providing the first evidence for direct interaction between members of the T-box gene family.

PubMed ID: 15634698
PMC ID: PMC1635804
Article link: Development.
Grant support: R01 HL075256 NHLBI NIH HHS , R01 HL075256-02 NHLBI NIH HHS , R01 HL075256 NHLBI NIH HHS

Genes referenced: actc1 actl6a hesx1 nkx2-5 ptpn11 tbx20 tbx5 tnni3 tpm1

Antibodies referenced: Tpm1 Ab1
Morpholinos referenced: tbx20 MO1 tbx20 MO2 tbx5 MO1 tbx5 MO2

References:
Baldini, 2004, Pubmed[+]


Article Images: [+] show captions

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