Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2.
Paris J
,
Le Guellec R
,
Couturier A
,
Le Guellec K
,
Omilli F
,
Camonis J
,
MacNeill S
,
Philippe M
.
???displayArticle.abstract???
Fertilization of Xenopus laevis eggs triggers a period of rapid cell division comprising 12 nearly synchronous mitoses. Protein synthesis is required for these divisions, and new proteins appear after fertilization. Others proteins however, which are synthesized in the unfertilized egg, are no longer made in the early embryo. To identify such proteins, a differential screen of an egg cDNA library gave nine clones corresponding to mRNAs that are deadenylylated soon after fertilization. The sequence of one of these clones (Eg1) revealed a high homology to p34cdc2, the kinase subunit of maturation-promoting factor. Only 12 amino acids in the deduced amino acid sequence were unique to Eg1 when its sequence was compared to all other known examples of cdc2. Despite this strong similarity, however, Eg1 was unable to complement a yeast cdc2- mutant in Schizosaccharomyces pombe or a cdc28 mutant of Saccharomyces cerevisiae. Four Eg1 transcripts, two major and two minor, were found in Xenopus oocytes and early embryos. These RNAs appeared very early (stage I) in oogenesis and their level remained constant until the midblastula transition, at which time they declined. Eg1 RNA is found in the poly(A)+ fraction of oocytes only between the time of meiotic maturation and fertilization--that is to say, in the unfertilized egg. At fertilization the RNA loses its poly(A) tail and at the same time leaves the polyribosomes.
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980, Pubmed
Auffray,
Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
1980,
Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
BRACHET,
THE EFFECTS OF ACTINOMYCIN D AND PUROMYCIN ON MORPHOGENESIS IN AMPHIBIAN EGGS AND ACETABULARIA MEDITERRANEA.
1964,
Pubmed
Banroques,
A mitochondrial RNA maturase gene transferred to the yeast nucleus can control mitochondrial mRNA splicing.
1986,
Pubmed
Bellé,
A possible role for Mg2+ ions in the induction of meiotic maturation of Xenopus oocyte.
1986,
Pubmed
,
Xenbase
Bravo,
Classes of proteins synthesized in oocytes, eggs, embryos, and differentiated tissues of Xenopus laevis.
1979,
Pubmed
,
Xenbase
Broach,
Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
1979,
Pubmed
Cisek,
Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2.
1989,
Pubmed
Colot,
Behavior of individual maternal pA+ RNAs during embryogenesis of Xenopus laevis.
1982,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Feinberg,
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
1984,
Pubmed
Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase
Gonzalez,
Variation among human 28S ribosomal RNA genes.
1985,
Pubmed
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Hindley,
Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases.
1984,
Pubmed
Hinnen,
Transformation of yeast.
1978,
Pubmed
Hsiao,
High-frequency transformation of yeast by plasmids containing the cloned yeast ARG4 gene.
1979,
Pubmed
Huarte,
Meiotic maturation of mouse oocytes triggers the translation and polyadenylation of dormant tissue-type plasminogen activator mRNA.
1987,
Pubmed
Jones,
Expression of the SV40 promoter in fission yeast: identification and characterization of an AP-1-like factor.
1988,
Pubmed
Krek,
Structure and developmental expression of the chicken CDC2 kinase.
1989,
Pubmed
Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
,
Pubmed
Lörincz,
Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes.
,
Pubmed
Maller,
Regulation of amphibian oocyte maturation.
1985,
Pubmed
,
Xenbase
McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase
Miake-Lye,
Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Moreau,
Detection of proto-oncogenes in the genome of the amphibian Xenopus laevis.
1989,
Pubmed
,
Xenbase
Murray,
Dominoes and clocks: the union of two views of the cell cycle.
1989,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Nurse,
Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe.
1976,
Pubmed
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase
Paris,
Changes in the polyadenylation of specific stable RNA during the early development of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Reed,
Mitotic role for the Cdc28 protein kinase of Saccharomyces cerevisiae.
1990,
Pubmed
Riabowol,
The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.
1989,
Pubmed
,
Xenbase
Rosenthal,
Widespread changes in the translation and adenylation of maternal messenger RNAs following fertilization of Spisula oocytes.
1987,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Smith,
Protein synthesis and messenger RNA levels along the animal-vegetal axis during early Xenopus development.
1986,
Pubmed
,
Xenbase
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Vassalli,
Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
1989,
Pubmed
Webb,
Weak bases inhibit cleavage and embryogenesis in amphibians and echinoderms.
1987,
Pubmed
,
Xenbase
