XB-ART-25386
Proc Natl Acad Sci U S A
December 1, 1990;
87
(23):
9153-7.
Recombinant yeast TFIID, a general transcription factor, mediates activation by the gene-specific factor USF in a chromatin assembly assay.
Meisterernst M
,
Horikoshi M
,
Roeder RG
.
Abstract
The TATA box-binding transcription factor
TFIID from Saccharomyces cerevisiae was tested for its ability to mediate regulatory factor functions both in a cell-free system reconstituted with other general initiation factors (purified from HeLa cells) and in a combined nucleosome assembly-transcription system. In the latter assay recombinant yeast
TFIID, expressed in and purified from bacteria, was sufficient to prevent nucleosome assembly-mediated repression and to mediate transcriptional enhancement of the adenovirus major late promoter by the gene-specific activator USF. In contrast, recombinant yeast
TFIID was unable to mediate activation by USF in the system reconstituted only with purified general factors. Under the same conditions a partially purified natural yeast
TFIID was able to mediate activation by both USF and
Sp1 (assayed with the human immunodeficiency virus promoter), but to a lesser extent than observed with a partially purified natural human
TFIID. The implications of these findings are discussed with respect to the structure of the yeast and human TATA factors and the possible involvement either of specific
TFIID modifications or of coactivators.
PubMed ID:
2251256
PMC ID:
PMC55122
Article link:
Proc Natl Acad Sci U S A
Grant support:
[+]
Species referenced:
Xenopus
Genes referenced:
sp1
taf6
tbp
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