Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor.
Straub RE
,
Frech GC
,
Joho RH
,
Gershengorn MC
.
???displayArticle.abstract???
Thyrotropin-releasing hormone (TRH) is an important extracellular regulatory molecule that functions as a releasing factor in the anterior pituitary gland and as a neurotransmitter/neuromodulator in the central and peripheral nervous systems. Binding sites for TRH are present in these tissues, but the TRH receptor (TRH-R) has not been purified from any source. Using Xenopus laevis oocytes in an expression cloning strategy, we have isolated a cDNA clone that encodes the mouse pituitaryTRH-R. This conclusion is based on the following evidence. Injection of sense RNA transcribed in vitro from this cDNA into Xenopus oocytes leads to expression of cell-surface receptors that bind TRH and the competitive antagonist chlordiazepoxide with appropriate affinities and that elicit electrophysiological responses to TRH with the appropriate concentration dependency. Antisense RNA inhibits the TRH response in Xenopus oocytes injected with RNA isolated from normal rat anterior pituitary glands. Finally, transfection of COS-1 cells with this cDNA leads to expression of receptors that bind TRH and chlordiazepoxide with appropriate affinities and that transduce TRH stimulation of inositol phosphate formation. The 3.8-kilobase mouse TRH-R cDNA encodes a protein of 393 amino acids that shows similarities to other guanine nucleotide-binding regulatory protein-coupled receptors.
Albert,
Thyrotropin-releasing hormone-induced spike and plateau in cytosolic free Ca2+ concentrations in pituitary cells. Relation to prolactin release.
1984, Pubmed
Albert,
Thyrotropin-releasing hormone-induced spike and plateau in cytosolic free Ca2+ concentrations in pituitary cells. Relation to prolactin release.
1984,
Pubmed
Bunzow,
Cloning and expression of a rat D2 dopamine receptor cDNA.
,
Pubmed
Cullen,
Use of eukaryotic expression technology in the functional analysis of cloned genes.
1987,
Pubmed
Del Sal,
The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing.
1989,
Pubmed
Dohlman,
Role of extracellular disulfide-bonded cysteines in the ligand binding function of the beta 2-adrenergic receptor.
1990,
Pubmed
Frech,
Construction of directional cDNA libraries enriched for full-length inserts in a transcription-competent vector.
1989,
Pubmed
Gershengorn,
Evidence for tight coupling of receptor occupancy by thyrotropin-releasing hormone to phospholipase C-mediated phosphoinositide hydrolysis in rat pituitary cells: use of chlordiazepoxide as a competitive antagonist.
1986,
Pubmed
Gershengorn,
Mechanism of signal transduction by TRH.
1989,
Pubmed
Hagen,
Evidence the yeast STE3 gene encodes a receptor for the peptide pheromone a factor: gene sequence and implications for the structure of the presumed receptor.
1986,
Pubmed
Hershey,
Molecular characterization of a functional cDNA encoding the rat substance P receptor.
1990,
Pubmed
Hinkle,
Thyrotropin-releasing hormone stimulates GTP hydrolysis by membranes from GH4C1 rat pituitary tumor cells.
1984,
Pubmed
Hinkle,
Pituitary TRH receptors.
1989,
Pubmed
Hökfelt,
Distribution of TRH-like immunoreactivity with special reference to coexistence with other neuroactive compounds.
1989,
Pubmed
Imai,
Measurement of lipid turnover in response to thyrotropin-releasing hormone.
1987,
Pubmed
Johnson,
Solubilization and characterization of thyrotropin-releasing hormone receptors from rat brain.
1984,
Pubmed
Johnson,
Receptor binding and characterization of TRH receptors.
1989,
Pubmed
Julius,
Molecular characterization of a functional cDNA encoding the serotonin 1c receptor.
1988,
Pubmed
,
Xenbase
Julius,
The 5HT2 receptor defines a family of structurally distinct but functionally conserved serotonin receptors.
1990,
Pubmed
Kikkawa,
The protein kinase C family: heterogeneity and its implications.
1989,
Pubmed
Klein,
A chemoattractant receptor controls development in Dictyostelium discoideum.
1988,
Pubmed
Kobilka,
Cloning, sequencing, and expression of the gene coding for the human platelet alpha 2-adrenergic receptor.
1987,
Pubmed
,
Xenbase
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lechan,
Pro-TRH gene expression and precursor peptides in rat brain. Observations by hybridization analysis and immunocytochemistry.
1989,
Pubmed
Liao,
Molecular cloning and expression of a fifth muscarinic acetylcholine receptor.
1989,
Pubmed
Libert,
Selective amplification and cloning of four new members of the G protein-coupled receptor family.
1989,
Pubmed
Martinez,
A flexible multiple sequence alignment program.
1988,
Pubmed
McFarland,
Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor family.
1989,
Pubmed
Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
Xenbase
O'Dowd,
Palmitoylation of the human beta 2-adrenergic receptor. Mutation of Cys341 in the carboxyl tail leads to an uncoupled nonpalmitoylated form of the receptor.
1989,
Pubmed
Oron,
Mechanism of membrane electrical response to thyrotropin-releasing hormone in Xenopus oocytes injected with GH3 pituitary cell messenger ribonucleic acid.
1987,
Pubmed
,
Xenbase
Oron,
Decreased TRH receptor mRNA activity precedes homologous downregulation: assay in oocytes.
1987,
Pubmed
,
Xenbase
Ovchinnikov YuA,
Octopus rhodopsin. Amino acid sequence deduced from cDNA.
1988,
Pubmed
Parmentier,
Molecular cloning of the thyrotropin receptor.
1989,
Pubmed
,
Xenbase
Pearson,
Rapid and sensitive sequence comparison with FASTP and FASTA.
1990,
Pubmed
Phillips,
Solubilization and characterization of pituitary thyrotropin-releasing hormone receptors.
1989,
Pubmed
Popot,
Tertiary structure of bacteriorhodopsin. Positions and orientations of helices A and B in the structural map determined by neutron diffraction.
1989,
Pubmed
Rapiejko,
Primary structure of a human protein which bears structural similarities to members of the rhodopsin/beta-adrenergic receptor family.
1988,
Pubmed
Rees,
Hydrophobic organization of membrane proteins.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schiffer,
Use of helical wheels to represent the structures of proteins and to identify segments with helical potential.
1967,
Pubmed
Seed,
An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2.
,
Pubmed
Sharif,
Sulfhydryl groups in receptor binding of thyrotropin-releasing hormone to rat amygdala.
1984,
Pubmed
Sharif,
Quantitative autoradiography of TRH receptors in discrete brain regions of different mammalian species.
1989,
Pubmed
Strader,
Conserved aspartic acid residues 79 and 113 of the beta-adrenergic receptor have different roles in receptor function.
1988,
Pubmed
Strader,
Identification of two serine residues involved in agonist activation of the beta-adrenergic receptor.
1989,
Pubmed
Strader,
Mutations that uncouple the beta-adrenergic receptor from Gs and increase agonist affinity.
1987,
Pubmed
Straub,
Thyrotropin-releasing hormone and GTP activate inositol trisphosphate formation in membranes isolated from rat pituitary cells.
1986,
Pubmed
Taylor,
Preparation of 3H-[3-M3-His2]TRH as an improved ligand for TRH receptors.
1981,
Pubmed
Yarden,
The avian beta-adrenergic receptor: primary structure and membrane topology.
1986,
Pubmed
Young,
Characterization of the rat mas oncogene and its high-level expression in the hippocampus and cerebral cortex of rat brain.
1988,
Pubmed
