Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-25430
Neuron December 1, 1990; 5 (6): 857-66.

Influence of the gamma subunit and expression system on acetylcholine receptor gating.

Lo DC , Pinkham JL , Stevens CF .


Abstract
We have developed a partial kinetic theory for the gating of murine nicotinic acetylcholine receptors (AChRs) expressed in Xenopus oocytes and have used this theory to characterize the role of the gamma subunit in single-channel behavior. Permeation and gating were found to be largely unaffected in AChRs produced in oocytes when the gamma subunit transcript was omitted from microinjections of AChR subunit RNAs. In contrast, marked changes in gating kinetics resulted when even very conservative single amino acid substitutions were introduced into the gamma subunit, indicating that the gamma subunit can have a large effect on AChR gating. We also found that channel openings were much prolonged when murine AChRs were expressed in BC3H-1 cells.

PubMed ID: 1702647
Article link: Neuron
Grant support: [+]


Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556