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Proc Natl Acad Sci U S A
2006 Jun 13;10324:8965-70. doi: 10.1073/pnas.0603327103.
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Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis.
Liu Z
,
Lin H
,
Ye S
,
Liu QY
,
Meng Z
,
Zhang CM
,
Xia Y
,
Margoliash E
,
Rao Z
,
Liu XJ
.
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Hydrogen peroxide (H(2)O(2)) is the major reactive oxygen species (ROS) produced in sperm. High concentrations of H(2)O(2) in sperm induce nuclear DNA fragmentation and lipid peroxidation and result in cell death. The respiratory chain of the mitochondrion is one of the most productive ROS generating systems in sperm, and thus the destruction of ROS in mitochondria is critical for the cell. It was recently reported that H(2)O(2) generated by the respiratory chain of the mitochondrion can be efficiently destroyed by the cytochrome c-mediated electron-leak pathway where the electron of ferrocytochrome c migrates directly to H(2)O(2) instead of to cytochrome c oxidase. In our studies, we found that mouse testis-specific cytochrome c (T-Cc) can catalyze the reduction of H(2)O(2) three times faster than its counterpart in somatic cells (S-Cc) and that the T-Cc heme has the greater resistance to being degraded by H(2)O(2). Together, these findings strongly imply that T-Cc can protect sperm from the damages caused by H(2)O(2). Moreover, the apoptotic activity of T-Cc is three to five times greater than that of S-Cc in a well established apoptosis measurement system using Xenopus egg extract. The dramatically stronger apoptotic activity of T-Cc might be important for the suicide of male germ cells, considered a physiological mechanism that regulates the number of sperm produced and eliminates those with damaged DNA. Thus, it is very likely that T-Cc has evolved to guarantee the biological integrity of sperm produced in mammalian testis.
Adinarayana,
Hydroxyl radical-induced strand break formation in single-stranded polynucleotides and single-stranded DNA in aqueous solution as measured by light scattering and by conductivity.
1988, Pubmed
Adinarayana,
Hydroxyl radical-induced strand break formation in single-stranded polynucleotides and single-stranded DNA in aqueous solution as measured by light scattering and by conductivity.
1988,
Pubmed
Aitken,
Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa.
1998,
Pubmed
Anderson,
Sperm competition: motility and the midpiece in primates.
2002,
Pubmed
Arama,
Caspase activity and a specific cytochrome C are required for sperm differentiation in Drosophila.
2003,
Pubmed
Baker,
Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa.
2005,
Pubmed
Barros,
H(2)O(2) generation in Saccharomyces cerevisiae respiratory pet mutants: effect of cytochrome c.
2003,
Pubmed
Bertini,
A structural model for the adduct between cytochrome c and cytochrome c oxidase.
2005,
Pubmed
Bielski,
A study of the reactivity of HO2/O2- with unsaturated fatty acids.
1983,
Pubmed
Brünger,
Crystallography & NMR system: A new software suite for macromolecular structure determination.
1998,
Pubmed
Cadenas,
Mitochondrial free radical generation, oxidative stress, and aging.
2000,
Pubmed
Cross,
Enzymic mechanisms of superoxide production.
1991,
Pubmed
Davies,
Redesign of the interior hydrophilic region of mitochondrial cytochrome c by site-directed mutagenesis.
1993,
Pubmed
Ferguson-Miller,
Definition of cytochrome c binding domains by chemical modification. III. Kinetics of reaction of carboxydinitrophenyl cytochromes c with cytochrome c oxidase.
1978,
Pubmed
Gavella,
NADH-dependent oxidoreductase (diaphorase) activity and isozyme pattern of sperm in infertile men.
1992,
Pubmed
Hake,
Utilization of an alternative transcription initiation site of somatic cytochrome c in the mouse produces a testis-specific cytochrome c mRNA.
1993,
Pubmed
Hess,
Immunoelectron microscopic localization of testicular and somatic cytochromes c in the seminiferous epithelium of the rat.
1993,
Pubmed
Jones,
Peroxidative breakdown of phospholipids in human spermatozoa, spermicidal properties of fatty acid peroxides, and protective action of seminal plasma.
1979,
Pubmed
Jones,
Improved methods for building protein models in electron density maps and the location of errors in these models.
1991,
Pubmed
Kumar,
MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment.
2004,
Pubmed
Lander,
Initial sequencing and analysis of the human genome.
2001,
Pubmed
Limbach,
Characterization of two Drosophila melanogaster cytochrome c genes and their transcripts.
1985,
Pubmed
Lo,
Structural and functional effects of multiple mutations at distal sites in cytochrome c.
1995,
Pubmed
Lu,
Nucleoplasmin regulates chromatin condensation during apoptosis.
2005,
Pubmed
,
Xenbase
MARGOLIASH,
PRIMARY STRUCTURE AND EVOLUTION OF CYTOCHROME C.
1963,
Pubmed
Mann,
Analysis of proteins and proteomes by mass spectrometry.
2001,
Pubmed
Mathai,
Stretch sensitivity of transmembrane mobility of hydrogen peroxide through voids in the bilayer. Role of cardiolipin.
1994,
Pubmed
Mills,
Cytochrome c: gene structure, homology and ancestral relationships.
1991,
Pubmed
Morehouse,
Effect of hydrogen peroxide on the initiation of microsomal lipid peroxidation.
1983,
Pubmed
Myer,
Ascorbate reduction of horse heart cytochrome c. A zero-energy reduction reaction.
1984,
Pubmed
Narisawa,
Testis-specific cytochrome c-null mice produce functional sperm but undergo early testicular atrophy.
2002,
Pubmed
Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed
Pande,
The arginines of cytochrome c. The reduction-binding site for 2,3-butanedione and ascorbate.
1980,
Pubmed
Patel,
Characterization of horse cytochrome c expressed in Escherichia coli.
2001,
Pubmed
Polevoda,
Cytochrome c methyltransferase, Ctm1p, of yeast.
2000,
Pubmed
Sanishvili,
The low ionic strength crystal structure of horse cytochrome c at 2.1 A resolution and comparison with its high ionic strength counterpart.
1995,
Pubmed
Sharlip,
Best practice policies for male infertility.
2002,
Pubmed
Sharma,
The reactive oxygen species-total antioxidant capacity score is a new measure of oxidative stress to predict male infertility.
1999,
Pubmed
Sharma,
Role of reactive oxygen species in male infertility.
1996,
Pubmed
Sherman,
The mutational alteration of the primary structure of yeast iso-1-cytochrome c.
1968,
Pubmed
Turrens,
Ubisemiquinone is the electron donor for superoxide formation by complex III of heart mitochondria.
1985,
Pubmed
Vera,
Mitochondria-dependent pathway is involved in heat-induced male germ cell death: lessons from mutant mice.
2004,
Pubmed
Wang,
Cytochrome C is a hydrogen peroxide scavenger in mitochondria.
2003,
Pubmed
Wang,
Oxidative stress is associated with increased apoptosis leading to spermatozoa DNA damage in patients with male factor infertility.
2003,
Pubmed
Waterhouse,
Cytochrome c maintains mitochondrial transmembrane potential and ATP generation after outer mitochondrial membrane permeabilization during the apoptotic process.
2001,
Pubmed
Wheeler,
Database resources of the National Center for Biotechnology Information.
2006,
Pubmed
Zhang,
The human genome has 49 cytochrome c pseudogenes, including a relic of a primordial gene that still functions in mouse.
2003,
Pubmed
Zhao,
Effect of cytochrome c on the generation and elimination of O2*- and H2O2 in mitochondria.
2003,
Pubmed
Zhao,
The operation of the alternative electron-leak pathways mediated by cytochrome c in mitochondria.
2004,
Pubmed
Zou,
Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3.
1997,
Pubmed