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EMBO J
1990 Oct 01;910:3233-40. doi: 10.1002/j.1460-2075.1990.tb07522.x.
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p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes.
Shuttleworth J
,
Godfrey R
,
Colman A
.
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The clone MO15 which codes for a 40 kd protein (p40MO15) with 40% amino acid identity to the human cdc2 protein kinase has been isolated from a Xenopus cDNA library using a synthetic oligonucleotide probe. MO15 mRNA is accumulated during oogenesis, becomes de-adenylated during meiotic maturation, and is degraded after the mid-blastula-transition stage of embryogenesis. Translation of p40MO15 is restricted to non-mature oocytes. Specific inhibition of p40MO15 synthesis in stage VI oocytes by antisense oligonucleotide depletion of MO15 mRNA increases the rate of progesterone induced H1 kinase activation and oocyte maturation. This effect can be reversed by subsequent injection of synthetic MO15 mRNA. These results suggest that p40MO15 is involved in negatively regulating meiosis.
Bailly,
p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.
1989, Pubmed
Bailly,
p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.
1989,
Pubmed
Booher,
Interaction between cdc13+ and cdc2+ in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2+ protein kinase.
1987,
Pubmed
Boyer,
Progesterone and cAMP-dependent protein kinase regulate in vivo the level of phosphorylation of two proteins (Mr 20,000 and Mr 32,000) in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Courchesne,
A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae.
1989,
Pubmed
Cyert,
Regulation of MPF activity in vitro.
1988,
Pubmed
,
Xenbase
Dale,
Developmental expression of the protein product of Vg1, a localized maternal mRNA in the frog Xenopus laevis.
1989,
Pubmed
,
Xenbase
Dash,
Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity.
1987,
Pubmed
,
Xenbase
Davis,
Monoclonal antibodies to mitotic cells.
1983,
Pubmed
,
Xenbase
Draetta,
Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement.
1988,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Dunphy,
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase.
1989,
Pubmed
,
Xenbase
Dworkin,
Changes in RNA titers and polyadenylation during oogenesis and oocyte maturation in Xenopus laevis.
1985,
Pubmed
,
Xenbase
Elion,
FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation.
1990,
Pubmed
Félix,
Cdc2 H1 kinase is negatively regulated by a type 2A phosphatase in the Xenopus early embryonic cell cycle: evidence from the effects of okadaic acid.
1990,
Pubmed
,
Xenbase
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gautier,
Dephosphorylation and activation of Xenopus p34cdc2 protein kinase during the cell cycle.
1989,
Pubmed
,
Xenbase
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Goris,
Okadaic acid, a specific protein phosphatase inhibitor, induces maturation and MPF formation in Xenopus laevis oocytes.
1989,
Pubmed
,
Xenbase
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Gurdon,
The croonian lecture, 1976. Egg cytoplasm and gene control in development.
1977,
Pubmed
,
Xenbase
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Hanks,
Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family.
1987,
Pubmed
Hindley,
Sequence of the cell division gene CDC2 from Schizosaccharomyces pombe; patterns of splicing and homology to protein kinases.
1984,
Pubmed
Horrell,
Transcript levels and translational control of hsp70 synthesis in Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Hunt,
Maturation promoting factor, cyclin and the control of M-phase.
1989,
Pubmed
Hyman,
Translational inactivation of ribosomal protein mRNAs during Xenopus oocyte maturation.
1988,
Pubmed
,
Xenbase
Kozak,
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
1984,
Pubmed
Kressmann,
Transcription of cloned tRNA gene fragments and subfragments injected into the oocyte nucleus of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Krieg,
In vitro RNA synthesis with SP6 RNA polymerase.
1987,
Pubmed
LaMarca,
Gonadotropins and the timing of progesterone-induced meiotic maturation of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Lee,
Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2.
,
Pubmed
Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
,
Xenbase
Maller,
Progesterone-stimulated meiotic cell division in Xenopus oocytes. Induction by regulatory subunit and inhibition by catalytic subunit of adenosine 3':5'-monophosphate-dependent protein kinase.
1977,
Pubmed
,
Xenbase
Maller,
Two-dimensional polyacrylamide gel analysis of changes in protein phosphorylation during maturation of Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Meijer,
Cyclin is a component of the sea urchin egg M-phase specific histone H1 kinase.
1989,
Pubmed
Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase
Moreno,
Substrates for p34cdc2: in vivo veritas?
1990,
Pubmed
Moreno,
Regulation of p34cdc2 protein kinase during mitosis.
1989,
Pubmed
Morla,
Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.
1989,
Pubmed
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Murray,
Cell biology: the cell cycle as a cdc2 cycle.
1989,
Pubmed
Nurse,
Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast.
1981,
Pubmed
Peter,
In vitro disassembly of the nuclear lamina and M phase-specific phosphorylation of lamins by cdc2 kinase.
1990,
Pubmed
Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed
Reynhout,
Response of large oocytes of Xenopus laevis to progesterone in vitro in relation to oocyte size and time after previous HCG-induced ovulation.
1975,
Pubmed
,
Xenbase
Rosenberg,
Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
1987,
Pubmed
Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase
Shuttleworth,
Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs.
1988,
Pubmed
,
Xenbase
Simon,
KIN28, a yeast split gene coding for a putative protein kinase homologous to CDC28.
1986,
Pubmed
Smith,
The induction of oocyte maturation: transmembrane signaling events and regulation of the cell cycle.
1989,
Pubmed
Spivack,
Microinjection of pp60v-src into Xenopus oocytes increases phosphorylation of ribosomal protein S6 and accelerates the rate of progesterone-induced meiotic maturation.
1984,
Pubmed
,
Xenbase
Studier,
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
1986,
Pubmed
Toh-e,
PHO85, a negative regulator of the PHO system, is a homolog of the protein kinase gene, CDC28, of Saccharomyces cerevisiae.
1988,
Pubmed
Wasserman,
Effects of cyclohexamide on a cytoplasmic factor initiating meiotic naturation in Xenopus oocytes.
1975,
Pubmed
,
Xenbase
Watanabe,
Specific proteolysis of the c-mos proto-oncogene product by calpain on fertilization of Xenopus eggs.
1989,
Pubmed
,
Xenbase
Woolf,
The stability, toxicity and effectiveness of unmodified and phosphorothioate antisense oligodeoxynucleotides in Xenopus oocytes and embryos.
1990,
Pubmed
,
Xenbase
