Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle.
Gard DL
,
Hafezi S
,
Zhang T
,
Doxsey SJ
.
???displayArticle.abstract???
Cycloheximide (500 micrograms/ml) rapidly arrests cleavage, spindle assembly, and cycles of an M-phase-specific histone kinase in early Xenopus blastulae. 2 h after cycloheximide addition, most cells contained two microtubule asters radiating from perinuclear microtubule organizing centers (MTOCs). In contrast, blastomeres treated with cycloheximide for longer periods (3-6 h) contained numerous microtubule asters and MTOCs. Immunofluorescence with an anticentrosome serum and EM demonstrated that the MTOCs in cycloheximide-treated cells were typical centrosomes, containing centrioles and pericentriolar material. We conclude that centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle. In addition, these observations suggest that Xenopus embryos contain sufficient material to assemble 1,000-2,000 centrosomes in the absence of normal protein synthesis.
Alvey,
An investigation of the centriole cycle using 3T3 and CHO cells.
1985, Pubmed
Alvey,
An investigation of the centriole cycle using 3T3 and CHO cells.
1985,
Pubmed
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Xenbase
Brinkley,
Tubulin assembly sites and the organization of cytoplasmic microtubules in cultured mammalian cells.
1981,
Pubmed
Brinkley,
Microtubule organizing centers.
1985,
Pubmed
Calarco-Gillam,
Centrosome development in early mouse embryos as defined by an autoantibody against pericentriolar material.
1983,
Pubmed
Cicirelli,
Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
DIRKSEN,
The presence of centrioles in artificially activated sea urchin eggs.
1961,
Pubmed
Dabauvalle,
Role of nuclear material in the early cell cycle of Xenopus embryos.
1988,
Pubmed
,
Xenbase
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
Forbes,
Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
1983,
Pubmed
,
Xenbase
Gard,
A polymer-dependent increase in phosphorylation of beta-tubulin accompanies differentiation of a mouse neuroblastoma cell line.
1985,
Pubmed
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
,
Xenbase
Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase
Hara,
A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
1980,
Pubmed
,
Xenbase
Kallenbach,
Continuous hypertonic conditions activate and promote the formation of new centrioles within cytasters in sea urchin eggs.
1982,
Pubmed
Kimelman,
The events of the midblastula transition in Xenopus are regulated by changes in the cell cycle.
1987,
Pubmed
,
Xenbase
Kuriyama,
Centriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy.
1981,
Pubmed
Labbe,
Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
1989,
Pubmed
,
Xenbase
Meijer,
Cyclic activation of histone H1 kinase during sea urchin egg mitotic divisions.
1988,
Pubmed
Miake-Lye,
Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Miki-Noumura,
Studies on the de novo formation of centrioles: aster formation in the activated eggs of sea urchin.
1977,
Pubmed
Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
,
Xenbase
Mitchison,
Microtubule assembly nucleated by isolated centrosomes.
,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Nagano,
Achromosomal cleavage of fertilized starfish eggs in the presence of aphidicolin.
1981,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Newport,
Regulation of the cell cycle during early Xenopus development.
1984,
Pubmed
,
Xenbase
Phillips,
Dependence of centriole formation on protein synthesis.
1976,
Pubmed
Picard,
Germinal vesicle components are not required for the cell-cycle oscillator of the early starfish embryo.
1988,
Pubmed
Raff,
Nuclear and cytoplasmic mitotic cycles continue in Drosophila embryos in which DNA synthesis is inhibited with aphidicolin.
1988,
Pubmed
Riabowol,
The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.
1989,
Pubmed
,
Xenbase
Robbins,
The centriole cycle in synchronized HeLa cells.
1968,
Pubmed
Sellitto,
Distribution of pericentriolar material in multipolar spindles induced by colcemid treatment in Chinese hamster ovary cells.
1988,
Pubmed
Sluder,
The reproduction of centrosomes: nuclear versus cytoplasmic controls.
1986,
Pubmed
Sluder,
Relationship between nuclear DNA synthesis and centrosome reproduction in sea urchin eggs.
1987,
Pubmed
Spiegelman,
Multiple sites for the initiation of microtubule assembly in mammalian cells.
1979,
Pubmed
Tuffanelli,
Anticentromere and anticentriole antibodies in the scleroderma spectrum.
1983,
Pubmed
Vandre,
Phosphoproteins are components of mitotic microtubule organizing centers.
1984,
Pubmed
Vorobjev,
Centrioles in the cell cycle. I. Epithelial cells.
1982,
Pubmed
Weisenberg,
In vitro polymerization of microtubules into asters and spindles in homogenates of surf clam eggs.
1975,
Pubmed
Woodland,
The synthesis and storage of histones during the oogenesis of Xenopus laevis.
1977,
Pubmed
,
Xenbase
