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Nucleosome assembly in vitro: separate histone transfer and synergistic interaction of native histone complexes purified from nuclei of Xenopus laevis oocytes.
Kleinschmidt JA
,
Seiter A
,
Zentgraf H
.
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High speed supernatants of Xenopus laevis oocyte nuclei efficiently assemble DNA into nucleosomes in vitro under physiological salt conditions. The assembly activity cofractionates with two histone complexes composed of the acidic protein N1/N2 in complex with histones H3 and H4, and nucleoplasmin in complex with histones H2B and H2A. Both histone complexes have been purified and their nucleosome assembly activities have been analysed separately and in combination. While the histones from the N1/N2 complexes are efficiently transferred to DNA and induce supercoils into relaxed circular plasmid DNA, the nucleoplasmin complexes show no supercoil induction, but can also transfer their histones to DNA. In combination, the complexes act synergistically in supercoil induction thereby increasing the velocity and the number of supercoils induced. Electron microscopic analysis of the reaction products shows fully packaged nucleoprotein structures with the typical nucleosomal appearance resulting in a compaction ratio of 2.8 under low ionic strength conditions. The high mobility group protein HMG-1, which is also present in the soluble nuclear homogenate from X. laevis oocytes, is not required for nucleosome core assembly. Fractionation experiments show that the synergistic effect in the supercoiling reaction can be exerted by histones H3 and H4 bound to DNA and the nucleoplasmin complexes alone. This indicates that it is not the synchronous action of both complexes which is required for nucleosome assembly, but that their cooperative action can be resolved into two steps: deposition of H3 and H4 from the N1/N2 complexes onto the DNA and completion of nucleosome core formation by addition of H2B and H2A from the nucleoplasmin complexes.
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974, Pubmed,
Xenbase
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974,
Pubmed
,
Xenbase
Almouzni,
Assembly of spaced chromatin promoted by DNA synthesis in extracts from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Aragay,
Association of nucleosome core particle DNA with different histone oligomers. Transfer of histones between DNA-(H2A,H2B) and DNA-(H3,H4) complexes.
1988,
Pubmed
Axel,
Specific sites of interaction between histones and DNA in chromatin.
1974,
Pubmed
Bonne,
Single-strand DNA binding protein from rat liver: interactions with supercoiled DNA.
1980,
Pubmed
Bonne-Andrea,
Rat liver HMG1: a physiological nucleosome assembly factor.
1984,
Pubmed
Bürglin,
Cloning of nucleoplasmin from Xenopus laevis oocytes and analysis of its developmental expression.
1987,
Pubmed
,
Xenbase
Cereghini,
Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.
1984,
Pubmed
Cotten,
Purification of a novel, nucleoplasmin-like protein from somatic nuclei.
1987,
Pubmed
,
Xenbase
Cremisi,
Chromatin-like structures in polyoma virus and simian virus 10 lytic cycle.
1975,
Pubmed
Crémisi,
Sequential assembly of newly synthesized histones on replicating SV40 DNA.
1980,
Pubmed
Dilworth,
Chromatin assembly in vitro and in vivo.
1988,
Pubmed
Dilworth,
Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts.
1987,
Pubmed
,
Xenbase
Dingwall,
Nucleoplasmin cDNA sequence reveals polyglutamic acid tracts and a cluster of sequences homologous to putative nuclear localization signals.
1987,
Pubmed
,
Xenbase
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Griffith,
Chromatin structure: deduced from a minichromosome.
1975,
Pubmed
Ishimi,
A protein which facilitates assembly of nucleosome-like structures in vitro in mammalian cells.
1983,
Pubmed
Ishimi,
Purification and initial characterization of a protein which facilitates assembly of nucleosome-like structure from mammalian cells.
1984,
Pubmed
,
Xenbase
Ishimi,
Binding mode of nucleosome-assembly protein (AP-I) and histones.
1987,
Pubmed
Jackson,
A new method for the isolation of replicative chromatin: selective deposition of histone on both new and old DNA.
1981,
Pubmed
Jackson,
Studies on histone organization in the nucleosome using formaldehyde as a reversible cross-linking agent.
1978,
Pubmed
Javaherian,
Nonhistone proteins HMG1 and HMG2 change the DNA helical structure.
1978,
Pubmed
Kleinschmidt,
Identification of domains involved in nuclear uptake and histone binding of protein N1 of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Kleinschmidt,
Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Kleinschmidt,
Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes.
1985,
Pubmed
,
Xenbase
Kleinschmidt,
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
1986,
Pubmed
,
Xenbase
Kleinschmidt,
High mobility group proteins of amphibian oocytes: a large storage pool of a soluble high mobility group-1-like protein and involvement in transcriptional events.
1983,
Pubmed
,
Xenbase
Laskey,
Assembly of SV40 chromatin in a cell-free system from Xenopus eggs.
1977,
Pubmed
,
Xenbase
Laskey,
Chromatin assembly and transcription in eggs and oocytes of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Laskey,
Nucleosome assembly.
1980,
Pubmed
,
Xenbase
Laskey,
Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA.
1978,
Pubmed
,
Xenbase
Müller,
Higher order structure of simian virus 40 chromatin.
1978,
Pubmed
Nelson,
Ribonucleic acid and other polyanions facilitate chromatin assembly in vitro.
1981,
Pubmed
Nelson,
Extracts of Drosophila embryos mediate chromatin assembly in vitro.
1979,
Pubmed
Oudet,
Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
1975,
Pubmed
Rodríguez-Campos,
Assembly and properties of chromatin containing histone H1.
1989,
Pubmed
,
Xenbase
Ruiz-Carrillo,
In vitro core particle and nucleosome assembly at physiological ionic strength.
1979,
Pubmed
Scheer,
Nucleosomal and supranucleosomal organization of transcriptionally inactive rDNA circles in Dytiscus oocytes.
1978,
Pubmed
Shimamura,
Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Smith,
Expression of a histone H1-like protein is restricted to early Xenopus development.
1988,
Pubmed
,
Xenbase
Smith,
Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro.
1989,
Pubmed
Stein,
Acidic polypeptides can assemble both histones and chromatin in vitro at physiological ionic strength.
1979,
Pubmed
Thomas,
An octamer of histones in chromatin and free in solution.
1975,
Pubmed
Waithe,
Histone synthesis by lymphocytes in G0 and G1.
1983,
Pubmed
Walker,
The primary structures of non-histone chromosomal proteins HMG 1 and 2.
1980,
Pubmed
Wedlich,
Occurrence of a species-specific nuclear antigen in the germ line of Xenopus and its expression from paternal genes in hybrid frogs.
1985,
Pubmed
,
Xenbase
West,
Histone 2A, a heteromorphous family of eight protein species.
1980,
Pubmed
Woodland,
Histone synthesis during the development of Xenopus.
1980,
Pubmed
,
Xenbase
Worcel,
Assembly of newly replicated chromatin.
1978,
Pubmed
Wu,
Separation of basal histone synthesis from S-phase histone synthesis in dividing cells.
1981,
Pubmed
Wu,
Patterns of histone variant synthesis can distinguish G0 from G1 cells.
1982,
Pubmed
Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Zentgraf,
Mitochondrial DNA arranged into chromatin-like structures after injection into amphibian oocyte nuclei.
1979,
Pubmed
,
Xenbase
Zentgraf,
Differences of supranucleosomal organization in different kinds of chromatin: cell type-specific globular subunits containing different numbers of nucleosomes.
1984,
Pubmed
van Dongen,
The accumulation of the maternal pool of histone H1A during oogenesis in Xenopus laevis.
1983,
Pubmed
,
Xenbase
