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XB-ART-261
Genes Dev June 1, 2006; 20 (11): 1441-6.

Combined ectopic expression of Pdx1 and Ptf1a/p48 results in the stable conversion of posterior endoderm into endocrine and exocrine pancreatic tissue.

Afelik S , Chen Y , Pieler T .


Abstract
Patterning of the embryonic endoderm into distinct sets of precursor cells involves the precisely regulated activities of key transcription regulators. Ectopic, pan-endodermal activation of XPtf1a/p48 during pancreas precursor cell stages of Xenopus embryogenesis results in an expansion of the pancreatic territory, precisely within the borders of XlHbox8 expression. A combination of both activities is sufficient to expand the pancreatic precursor cell population also into more posterior portions of the endoderm. Both treatments result in the formation of a giant pancreas that persists up to late tadpole stages of development and carries both supernumerary endocrine and exocrine cells. A combination of XPtf1a/p48 and XlHbox8 is thus sufficient to convert nonpancreatic endodermal cells into pancreatic precursor cells.

PubMed ID: 16751182
PMC ID: PMC1475757
Article link: Genes Dev


Species referenced: Xenopus laevis
Genes referenced: amy2a darmin egr2 en2 foxa1 gcg hhex ins pdia2 pdx1 ptf1a
Antibodies: Gcg Ab3 Ins Ab3
Morpholinos: pdx1 MO1 ptf1a MO3

Phenotypes: Xla Wt + pdx1 MO (fig.S4.2) [+]

Article Images: [+] show captions
References [+] :
Afelik, Pancreatic protein disulfide isomerase (XPDIp) is an early marker for the exocrine lineage of the developing pancreas in Xenopus laevis embryos. 2003, Pubmed, Xenbase