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J Cell Biol December 6, 2004; 167 (5): 813-8.

The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles.

Miyamoto DT , Perlman ZE , Burbank KS , Groen AC , Mitchison TJ .

Although mitotic and meiotic spindles maintain a steady-state length during metaphase, their antiparallel microtubules slide toward spindle poles at a constant rate. This "poleward flux" of microtubules occurs in many organisms and may provide part of the force for chromosome segregation. We use quantitative image analysis to examine the role of the kinesin Eg5 in poleward flux in metaphase Xenopus laevis egg extract spindles. Pharmacological inhibition of Eg5 results in a dose-responsive slowing of flux, and biochemical depletion of Eg5 significantly decreases the flux rate. Our results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles.

PubMed ID: 15583027
PMC ID: PMC2172449
Article link: J Cell Biol

Genes referenced: arhgef7 dctn2 kif11

Article Images: [+] show captions
References [+] :
Brust-Mascher, Microtubule flux and sliding in mitotic spindles of Drosophila embryos. 2002, Pubmed

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