Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
DNA superhelicity enhances the assembly of transcriptionally active chromatin in vitro.
Sekiguchi JM
,
Kmiec EB
.
???displayArticle.abstract???
Using an in vitro chromatin assembly system, we analyzed the influence of DNA superhelicity on the development of transcriptionally active minichromosomes. Plasmid DNA molecules containing either a Xenopus borealis 5S RNA gene or an X. laevis methionine tRNA gene were utilized as templates for the assembly of chromatin. Both plasmids were processed into active minichromosomes if introduced as supercoiled molecules into the extract (S-150). The degree of superhelicity is a determining factor in the assembly of active chromatin. Molecules containing varying superhelical densities were processed into minichromosomes with different transcriptional activities. The absence of supercoils leads to the assembly of chromatin with substantially lower transcriptional activity. Assembled minichromosomes are stable enough to be isolated by sucrose gradient centrifugation while retaining their transcriptional phenotype. The formation of nucleosomes with a periodic spacing occurred with the same efficiency and to the same degree regardless of the initial DNA topology. Hence, a determining factor in the development of transcriptionally active chromatin may be the initial superhelicity of the DNA molecule to which activator (trans-acting factors) or repressor (histones) proteins bind. Once the chromatin assembly process has begun, the transcriptional activity of the resulting minichromosome may already have been determined.
Ambrose,
The flexibility and topology of simian virus 40 DNA in minichromosomes.
1987, Pubmed
Ambrose,
The flexibility and topology of simian virus 40 DNA in minichromosomes.
1987,
Pubmed
Brown,
The role of stable complexes that repress and activate eucaryotic genes.
1984,
Pubmed
,
Xenbase
Choder,
In vitro transcribed SV40 minichromosomes, as the bulk minichromosomes, have a low level of unconstrained negative supercoils.
1988,
Pubmed
Cozzarelli,
Purified RNA polymerase III accurately and efficiently terminates transcription of 5S RNA genes.
1983,
Pubmed
,
Xenbase
Dezélée,
Role of deoxyribonucleic acid-ribonucleic acid hybrids in eukaryotes. Synthetic ribo- and deoxyribopolynucleotides as template for yeast ribonucleic acid polymerase B (or II).
1974,
Pubmed
Dunn,
An operator at -280 base pairs that is required for repression of araBAD operon promoter: addition of DNA helical turns between the operator and promoter cyclically hinders repression.
1984,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Gargiulo,
Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors.
1984,
Pubmed
,
Xenbase
Garner,
Effects of DNA supercoiling on the topological properties of nucleosomes.
1987,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase
Han,
Novobiocin blocks the Drosophila heat shock response.
1985,
Pubmed
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Huang,
Delineation of DNA sequences that are important for in vitro transcription from the adenovirus EIIa late promoter.
1988,
Pubmed
Kmiec,
The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in Xenopus oocyte extracts.
1985,
Pubmed
,
Xenbase
Kmiec,
The role of DNA-mediated transfer of TFIIIA in the concerted gyration and differential activation of the Xenopus 5S RNA genes.
1986,
Pubmed
,
Xenbase
Mizushima-Sugano,
Cell-type-specific transcription of an immunoglobulin kappa light chain gene in vitro.
1986,
Pubmed
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Peterson,
Context-dependent gene expression: cis-acting negative effects of specific procaryotic plasmid sequences on eucaryotic genes.
1987,
Pubmed
Petryniak,
Topological characterization of the simian virus 40 transcription complex.
1987,
Pubmed
,
Xenbase
Pruitt,
Effect of topological constraint on transcription of ribosomal DNA in Xenopus oocytes. Comparison of plasmid and endogenous genes.
1984,
Pubmed
,
Xenbase
Razvi,
A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA.
1983,
Pubmed
,
Xenbase
Reeves,
Transcriptionally active chromatin.
1984,
Pubmed
Ruberti,
Mechanism of chromatin assembly in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase
Sekiguchi,
Changes in DNA topology can modulate in vitro transcription of certain RNA polymerase III genes.
1989,
Pubmed
,
Xenbase
Simpson,
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure.
1985,
Pubmed
Sinden,
Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: analogous measurements in insect and human cells.
1980,
Pubmed
Singleton,
The facile generation of covalently closed, circular DNAs with defined negative superhelical densities.
1982,
Pubmed
Tabuchi,
DNA supercoiling facilitates formation of the transcription initiation complex on the fibroin gene promoter.
1988,
Pubmed
Wang,
DNA topoisomerases.
1985,
Pubmed
Weintraub,
Expression of transfected DNA depends on DNA topology.
1986,
Pubmed
Weintraub,
Assembly and propagation of repressed and depressed chromosomal states.
1985,
Pubmed
Weischet,
Differences in the nuclease sensitivity between the two alleles of the immunoglobulin kappa light chain genes in mouse liver and myeloma nuclei.
1982,
Pubmed
Workman,
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
1987,
Pubmed
,
Xenbase
