Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1989 Nov 01;911:4941-50. doi: 10.1128/mcb.9.11.4941-4950.1989.
Show Gene links
Show Anatomy links
Human transcription factor TFIIIC2 specifically interacts with a unique sequence in the Xenopus laevis 5S rRNA gene.
Fradkin LG
,
Yoshinaga SK
,
Berk AJ
,
Dasgupta A
.
???displayArticle.abstract???
Transcription factor TFIIIC2 derived from human cells is required for tRNA-type gene transcription and binds with high affinity to the essential B-box promoter element of tRNA-type genes. Although 5S rRNA genes contain no homology with the tRNA-type gene B box, we show that TFIIIC2 is also required for Xenopus laevis 5S rRNA gene transcription. TFIIIC2 protected an approximately 30-base-pair (-10 to +18) region of a Xenopus 5S rRNA gene from DNase I digestion. This region, which spanned the transcription start site, included sequences that are highly conserved among eucaryotic 5S rRNA genes and have no homology with the B-box sequence of tRNA genes. Mutation of the TFIIIC2-binding site reduced transcription of the 5S rRNA gene by a factor of 10 in HeLa cell extracts. Methylation of C residues within the TFIIIC2-binding site interfered with binding of TFIIIC2. These results suggest a role of the TFIIIC2-binding sequence in 5S rRNA gene transcription. In addition, the 5S rRNA gene binding site and the tRNA-type gene B-box sequence did not compete with each other for binding to TFIIIC2 any better than did an unrelated DNA sequence, indicating that TFIIIC2 interacts with 5S rRNA genes and tRNA-type genes through separate DNA-binding domains or polypeptides.
Baker,
Effects of tRNATyr point mutations on the binding of yeast RNA polymerase III transcription factor C.
1986, Pubmed
Baker,
Effects of tRNATyr point mutations on the binding of yeast RNA polymerase III transcription factor C.
1986,
Pubmed
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Boulanger,
DNA-binding properties and characterization of human transcription factor TFIIIC2.
1987,
Pubmed
Braun,
Multiple states of protein-DNA interaction in the assembly of transcription complexes on Saccharomyces cerevisiae 5S ribosomal RNA genes.
1989,
Pubmed
Camier,
A split binding site for transcription factor tau on the tRNA3Glu gene.
1985,
Pubmed
Carcamo,
Factors involved in specific transcription by mammalian RNA polymerase II. Role of factors IID and MLTF in transcription from the adenovirus major late and IVa2 promoters.
1989,
Pubmed
Carey,
Analysis of RNA polymerase III transcription complexes by gel filtration.
1986,
Pubmed
,
Xenbase
Ciliberto,
Common and interchangeable elements in the promoters of genes transcribed by RNA polymerase iii.
1983,
Pubmed
,
Xenbase
Davidson,
The HeLa cell protein TEF-1 binds specifically and cooperatively to two SV40 enhancer motifs of unrelated sequence.
1988,
Pubmed
Dean,
Ordering promoter binding of class III transcription factors TFIIIC1 and TFIIIC2.
1988,
Pubmed
Dean,
Separation of TFIIIC into two functional components by sequence specific DNA affinity chromatography.
1987,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Fowlkes,
Transcriptional control regions of the adenovirus VAI RNA gene.
1980,
Pubmed
Fradkin,
Inhibition of host cell RNA polymerase III-mediated transcription by poliovirus: inactivation of specific transcription factors.
1987,
Pubmed
Fuhrman,
HeLa cell RNA polymerase III transcription factors. Functional characterization of a fraction identified by its activity in a second template rescue assay.
1984,
Pubmed
Gabrielsen,
Two polypeptide chains in yeast transcription factor tau interact with DNA.
1989,
Pubmed
Galli,
Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
1981,
Pubmed
,
Xenbase
Geiduschek,
Transcription by RNA polymerase III.
1988,
Pubmed
Hart,
Structure and organization of a mammalian 5 S gene cluster.
1982,
Pubmed
,
Xenbase
Hayes,
A protein-protein interaction is essential for stable complex formation on a 5 S RNA gene.
1989,
Pubmed
,
Xenbase
Hoey,
Divergent homeo box proteins recognize similar DNA sequences in Drosophila.
1988,
Pubmed
Kadonaga,
Affinity purification of sequence-specific DNA binding proteins.
1986,
Pubmed
Lassar,
Transcription of class III genes: formation of preinitiation complexes.
1983,
Pubmed
,
Xenbase
Majowski,
A split binding site for TFIIIC on the Xenopus 5S gene.
1987,
Pubmed
,
Xenbase
Marzouki,
Selective proteolysis defines two DNA binding domains in yeast transcription factor tau.
,
Pubmed
Maxam,
Promotor region for yeast 5S ribosomal RNA.
1977,
Pubmed
Moitoso de Vargas,
Autonomous DNA binding domains of lambda integrase recognize two different sequence families.
1988,
Pubmed
Morton,
Silkworm 5S RNA and alanine tRNA genes share highly conserved 5' flanking and coding sequences.
1982,
Pubmed
Ottonello,
The properties of a new polymerase III transcription factor reveal that transcription complexes can assemble by more than one pathway.
1987,
Pubmed
Peterson,
Characterization of two xenopus somatic 5S DNAs and one minor oocyte-specific 5S DNA.
1980,
Pubmed
,
Xenbase
Pfeifer,
Yeast HAP1 activator binds to two upstream activation sites of different sequence.
1987,
Pubmed
Pieler,
Point mutational analysis of the Xenopus laevis 5S gene promoter.
1985,
Pubmed
,
Xenbase
Pieler,
Functional domains of the Xenopus laevis 5S gene promoter.
1985,
Pubmed
,
Xenbase
Pieler,
The 5S gene internal control region is composed of three distinct sequence elements, organized as two functional domains with variable spacing.
1987,
Pubmed
,
Xenbase
Razvi,
A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA.
1983,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase
Sands,
TFIIIA binds to different domains of 5S RNA and the Xenopus borealis 5S RNA gene.
1987,
Pubmed
,
Xenbase
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Segall,
Assembly of a yeast 5 S RNA gene transcription complex.
1986,
Pubmed
Seifart,
Purification of human transcription factor IIIA and its interaction with a chemically synthesized gene encoding human 5 S rRNA.
1989,
Pubmed
,
Xenbase
Setzer,
Formation and stability of the 5 S RNA transcription complex.
1985,
Pubmed
,
Xenbase
Sharp,
Transcription of the Drosophila melanogaster 5S RNA gene requires an upstream promoter and four intragenic sequence elements.
1988,
Pubmed
,
Xenbase
Smith,
Domains of the positive transcription factor specific for the Xenopus 5S RNA gene.
1984,
Pubmed
,
Xenbase
Stillman,
Effects of temperature and single-stranded DNA on the interaction of an RNA polymerase III transcription factor with a tRNA gene.
1985,
Pubmed
Stillman,
Differential binding of a S. cerevisiae RNA polymerase III transcription factor to two promoter segments of a tRNA gene.
1984,
Pubmed
Taylor,
Characterization of factors and DNA sequences required for accurate transcription of the Saccharomyces cerevisiae 5 S RNA gene.
1985,
Pubmed
,
Xenbase
Tschudi,
Sequence and heterogeneity in the 5S RNA gene cluster of Drosophila melanogaster.
1980,
Pubmed
Valenzuela,
Nucleotide sequence of the yeast 5S ribosomal RNA gene and adjacent putative control regions.
1977,
Pubmed
Wolffe,
A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
1986,
Pubmed
,
Xenbase
Wolffe,
Transcription fraction TFIIIC can regulate differential Xenopus 5S RNA gene transcription in vitro.
1988,
Pubmed
,
Xenbase
Wolters,
Compilation of 5S rRNA and 5S rRNA gene sequences.
1988,
Pubmed
Wormington,
Onset of 5 S RNA gene regulation during Xenopus embryogenesis.
1983,
Pubmed
,
Xenbase
Wu,
Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
1978,
Pubmed
Yoshinaga,
Resolution of human transcription factor TFIIIC into two functional components.
1987,
Pubmed
Yoshinaga,
Purification and characterization of transcription factor IIIC2.
1989,
Pubmed
