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XB-ART-26444
Nucleic Acids Res October 25, 1989; 17 (20): 8171-84.

Nuclear factors specifically bind to upstream sequences of a Xenopus laevis ribosomal protein gene promoter.

Carnevali F , La Porta C , Ilardi V , Beccari E .


Abstract
The upstream region of the Xenopus laevis L14 ribosomal protein gene was deleted starting from the 5'' extremity in order to define the promoter length necessary to express a linked reporter CAT gene. The functional analysis indicated that a sequence located between -63 and -49 from the capsite is important for an efficient promoter activity. Band shift and ExoIII protection assays evidenced the binding to this region of a factor, called XrpFI, present in the crude nuclear extract from X.laevis oocytes. Methylation interference analysis localized the contacts in the G residues belonging to a short box, 5'' CTTCC 3'', positioned between -53 and -49 from the capsite. An additional factor, XrpFII, makes contacts with the sequence 5''GCCTGTTCGCC 3'' located between -27 and -17 from the capsite. The deletion mutant still containing this sequence is poorly transcribed, but resumes activity when a short fragment containing the binding site for factor XrpFI is cloned in an upstream position.

PubMed ID: 2682523
PMC ID: PMC334956
Article link: Nucleic Acids Res


Species referenced: Xenopus
Genes referenced: cat.2 gabpa

References [+] :
Atchison, Localization of transcriptional regulatory elements and nuclear factor binding sites in mouse ribosomal protein gene rpL32. 1989, Pubmed