Shastry BS (1989), Immunological studies on gamma crystallins from...

XB-ART-26522

Exp Eye Res 1989 Sep 01;493:361-9.

Show Gene links Show Anatomy links

Immunological studies on gamma crystallins from Xenopus: localization, tissue specificity and developmental expression of proteins.

Shastry BS .

???displayArticle.abstract???
In an effort to understand the spatio-temporal regulation of crystallins and their genes during lens development, the gamma crystallins from the frog lens have been isolated, purified and characterized. Using an immunological approach, they were found to be localized exclusively in the lens fiber cells and were not detected in any other lens cells or non-lens tissues including mature oocytes. During embryogenesis, the antigens were first detected in stage 25 embryos (but not in stage 20 embryos). Their level first decreased and then increased during subsequent stages of development. A different member of the family was also found to be expressed during later stages of embryogenesis.

???displayArticle.pubmedLink??? 2792233
???displayArticle.link??? Exp Eye Res
???displayArticle.grants??? [+]

Species referenced: Xenopus laevis
Genes referenced: crygdl.30 crygdl.43

???attribute.lit??? ???displayArticles.show???

	FIG. 1. Elution profile of Xeaopuu eye lens extract from an ultrogel AcA 34 column. The insert shows the SDS gel electrophoresis of pooled fractions from peak II and III. The lanes were overloaded to detect possible contaminating proteins. The gel was stained with (‘oomassie Blue and the molecular weight markers (M) are shown on the left.

	FIG. 3. Immunological analysis of the localization of y-cryAallins in the adult lens. Panel A: The transmission electron micrograph of a section of lrns labeled with immunogold. showing the capsule (CP), epithelium (EP) and nuclear regions (N): which are negatlw for the gold particles ( x 2500). The arrows in the fiber area (F) shows gold particles. Panel B: The transmission electron micrograph of the same section of the lens but showing the fiber region where the dense distribution of the gold particles is observed (x 24000). Panel (!: Immunofluorescence micrograph of adult ,\‘e~~~pus lens treated with ycrystalhn antiserum showing very dense fluoresorr~w in the tiber region (P) and negative staining in the capsule (CP) area.