Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Complex formation between influenza virus polymerase proteins expressed in Xenopus oocytes.
Digard P
,
Blok VC
,
Inglis SC
.
???displayArticle.abstract???
All three influenza virus polymerase (P) proteins were expressed in Xenopus oocytes from microinjected in vitro transcribed mRNA analogs, with yields of up to 100 ng per oocyte. To examine the functional state of the Xenopus-expressed P proteins, the polypeptides were tested for their ability to form stable complexes with each other. As seen in virus-infected cells, all three P proteins associated into an immunoprecipitable complex, suggesting that the system has considerable promise for the reconstruction of an active influenza RNA polymerase. Examination of the ability of paired combinations of the P proteins to associate indicated that PB1 contained independent binding sites for PB2 and PA, and so probably formed the backbone of the complex. Sedimentation analysis of free and complexed P proteins indicated that PB1 and PB2 did not exist as free monomers, and that similarly, complexes of all three P proteins did not simply consist of one copy of each protein. The heterodisperse sedimentation rate seen for complexes of all three P proteins did not appear to result from their binding to RNA, suggesting the incorporation of additional polypeptides in the polymerase complex.
Akkina,
Intracellular localization of the viral polymerase proteins in cells infected with influenza virus and cells expressing PB1 protein from cloned cDNA.
1987, Pubmed
Akkina,
Intracellular localization of the viral polymerase proteins in cells infected with influenza virus and cells expressing PB1 protein from cloned cDNA.
1987,
Pubmed
Bishop,
Transcription of the influenza ribonucleic acid genome by a virion polymerase. I. Optimal conditions for in vitro activity of the ribonucleic acid-dependent ribonucleic acid polymerase.
1971,
Pubmed
Braam,
Molecular model of a eucaryotic transcription complex: functions and movements of influenza P proteins during capped RNA-primed transcription.
1983,
Pubmed
Braam-Markson,
Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector.
1985,
Pubmed
Brierley,
An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV.
1987,
Pubmed
Detjen,
The three influenza virus polymerase (P) proteins not associated with viral nucleocapsids in the infected cell are in the form of a complex.
1987,
Pubmed
Drummond,
The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Hay,
Influenza virus messenger RNAs are incomplete transcripts of the genome RNAs.
1977,
Pubmed
Hay,
Transcription of the influenza virus genome.
1977,
Pubmed
Herz,
Influenza virus, an RNA virus, synthesizes its messenger RNA in the nucleus of infected cells.
1981,
Pubmed
Inglis,
Polypeptides specified by the influenza virus genome I. Evidence for eight distinct gene products specified by fowl plague virus.
1976,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Krystal,
Expression of the three influenza virus polymerase proteins in a single cell allows growth complementation of viral mutants.
1986,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Plotch,
Transfer of 5'-terminal cap of globin mRNA to influenza viral complementary RNA during transcription in vitro.
1979,
Pubmed
St Angelo,
Two of the three influenza viral polymerase proteins expressed by using baculovirus vectors form a complex in insect cells.
1987,
Pubmed
Stanley,
Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.
1984,
Pubmed
Szewczyk,
Purification, thioredoxin renaturation, and reconstituted activity of the three subunits of the influenza A virus RNA polymerase.
1988,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
