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Nucleic Acids Res March 11, 1989; 17 (5): 1845-63.

Anti-OTF-1 antibodies inhibit NFIII stimulation of in vitro adenovirus DNA replication.

Pruijn JM , van der Vliet PC , Dathan NA , Mattaj IW .

HeLa cell OTF-1 has been purified on the basis of its DNA binding activity and used to raise a polyclonal rabbit antiserum. This antiserum is shown to recognize both native and denatured OTF-1 from both human and a similar protein from Xenopus culture cells, but to react either more weakly or not at all with the lymphoid cell-specific OTF-2. Separately, NFIII has been purified on the basis of its ability to stimulate Adenovirus DNA replication in vitro. On denaturing polyacrylamide gels OTF-1 and NFIII exhibit identical mobility. Anti-OTF-1 antiserum recognizes NFIII and neutralizes its stimulatory effect on DNA replication. Moreover, OTF-1 can functionally replace NFIII. Taken together with previously published DNA binding data, this indicates that OTF-1 and NFIII are either very closely related or identical.

PubMed ID: 2928110
PMC ID: PMC317527
Article link: Nucleic Acids Res

Species referenced: Xenopus laevis
Genes referenced: pou2f1

References [+] :
Ares, Orientation-dependent transcriptional activator upstream of a human U2 snRNA gene. 1985, Pubmed, Xenbase