Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Identification of adipocyte adhesion molecule (ACAM), a novel CTX gene family, implicated in adipocyte maturation and development of obesity.
Eguchi J
,
Wada J
,
Hida K
,
Zhang H
,
Matsuoka T
,
Baba M
,
Hashimoto I
,
Shikata K
,
Ogawa N
,
Makino H
.
???displayArticle.abstract???
Few cell adhesion molecules have been reported to be expressed in mature adipocytes, and the significance of cell adhesion process in adipocyte biology is also unknown. In the present study, we identified ACAM (adipocyte adhesion molecule), a novel homologue of the CTX (cortical thymocyte marker in Xenopus) gene family. ACAM cDNA was isolated during PCR-based cDNA subtraction, and its mRNA was shown to be up-regulated in WATs (white adipose tissues) of OLETF (Otsuka Long-Evans Tokushima fatty) rats, an animal model for Type II diabetes and obesity. ACAM, 372 amino acids in total, has a signal peptide, V-type (variable) and C2-type (constant) Ig domains, a single transmembrane segment and a cytoplasmic tail. The amino acid sequence in rat is highly homologous to mouse (94%) and human (87%). ACAM mRNA was predominantly expressed in WATs in OLETF rats, and increased with the development of obesity until 30 weeks of age, which is when the peak of body mass is reached. Western blot analysis revealed that ACAM protein, approx. 45 kDa, was associated with plasma membrane fractions of mature adipocytes isolated from mesenteric and subdermal adipose deposits of OLETF rats. Up-regulation of ACAM mRNAs in obesity was also shown in WATs of genetically obese db/db mice, diet-induced obese ICR mice and human obese subjects. In primary cultured mouse and human adipocytes, ACAM mRNA expression was progressively up-regulated during differentiation. Several stably transfected Chinese-hamster ovary K1 cell lines were established, and the quantification of ACAM mRNA and cell aggregation assay revealed that the degree of homophilic aggregation correlated well with ACAM mRNA expression. In summary, ACAM may be the critical adhesion molecule in adipocyte differentiation and development of obesity.
Arrate,
Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor.
2001, Pubmed
Arrate,
Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor.
2001,
Pubmed
Bergelson,
Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5.
1997,
Pubmed
Chrétien,
CTX, a novel molecule specifically expressed on the surface of cortical thymocytes in Xenopus.
1996,
Pubmed
,
Xenbase
Chrétien,
CTX, a Xenopus thymocyte receptor, defines a molecular family conserved throughout vertebrates.
1998,
Pubmed
,
Xenbase
Cohen,
The coxsackievirus and adenovirus receptor is a transmembrane component of the tight junction.
2001,
Pubmed
Cunningham,
JAM2 interacts with alpha4beta1. Facilitation by JAM3.
2002,
Pubmed
Ebnet,
The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: a possible role for JAMs in endothelial cell polarity.
2003,
Pubmed
Freimuth,
Coxsackievirus and adenovirus receptor amino-terminal immunoglobulin V-related domain binds adenovirus type 2 and fiber knob from adenovirus type 12.
1999,
Pubmed
Gregoire,
Understanding adipocyte differentiation.
1998,
Pubmed
Heath,
The human A33 antigen is a transmembrane glycoprotein and a novel member of the immunoglobulin superfamily.
1997,
Pubmed
Hida,
Identification of genes specifically expressed in the accumulated visceral adipose tissue of OLETF rats.
2000,
Pubmed
Hirata Ki,
Cloning of an immunoglobulin family adhesion molecule selectively expressed by endothelial cells.
2001,
Pubmed
Johnstone,
Characterization of mouse A33 antigen, a definitive marker for basolateral surfaces of intestinal epithelial cells.
2000,
Pubmed
,
Xenbase
Jones,
Regulation of gene expression and cell function by extracellular matrix.
1993,
Pubmed
Joost,
Subcellular fractionation of adipocytes and 3T3-L1 cells.
2001,
Pubmed
Kannel,
Regional obesity and risk of cardiovascular disease; the Framingham Study.
1991,
Pubmed
Kawano,
Spontaneous long-term hyperglycemic rat with diabetic complications. Otsuka Long-Evans Tokushima Fatty (OLETF) strain.
1992,
Pubmed
Kostrewa,
X-ray structure of junctional adhesion molecule: structural basis for homophilic adhesion via a novel dimerization motif.
2001,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Liang,
Vascular endothelial-junctional adhesion molecule (VE-JAM)/JAM 2 interacts with T, NK, and dendritic cells through JAM 3.
2002,
Pubmed
Lyon,
Minireview: adiposity, inflammation, and atherogenesis.
2003,
Pubmed
Martìn-Padura,
Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration.
1998,
Pubmed
Matsuzawa,
Molecular mechanism of metabolic syndrome X: contribution of adipocytokines adipocyte-derived bioactive substances.
1999,
Pubmed
Mitaku,
Amphiphilicity index of polar amino acids as an aid in the characterization of amino acid preference at membrane-water interfaces.
2002,
Pubmed
Nagai,
Coxsackievirus and adenovirus receptor, a tight junction membrane protein, is expressed in glomerular podocytes in the kidney.
2003,
Pubmed
Ostermann,
JAM-1 is a ligand of the beta(2) integrin LFA-1 involved in transendothelial migration of leukocytes.
2002,
Pubmed
Palmeri,
Vascular endothelial junction-associated molecule, a novel member of the immunoglobulin superfamily, is localized to intercellular boundaries of endothelial cells.
2000,
Pubmed
Phillips,
Narrowing the critical region within 11q24-qter for hypoplastic left heart and identification of a candidate gene, JAM3, expressed during cardiogenesis.
2002,
Pubmed
Raschperger,
CLMP, a novel member of the CTX family and a new component of epithelial tight junctions.
2004,
Pubmed
Rexrode,
Abdominal adiposity and coronary heart disease in women.
1998,
Pubmed
Santoso,
The junctional adhesion molecule 3 (JAM-3) on human platelets is a counterreceptor for the leukocyte integrin Mac-1.
2002,
Pubmed
Shin,
Relative abundance of different cadherins defines differentiation of mesenchymal precursors into osteogenic, myogenic, or adipogenic pathways.
2000,
Pubmed
Stevens,
The effect of age on the association between body-mass index and mortality.
1998,
Pubmed
Strausberg,
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
2002,
Pubmed
Suzu,
Molecular cloning of a novel immunoglobulin superfamily gene preferentially expressed by brain and testis.
2002,
Pubmed
Takeichi,
Functional correlation between cell adhesive properties and some cell surface proteins.
1977,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Tomko,
HCAR and MCAR: the human and mouse cellular receptors for subgroup C adenoviruses and group B coxsackieviruses.
1997,
Pubmed
Tsuchiyama,
Efficacy of galectins in the amelioration of nephrotoxic serum nephritis in Wistar Kyoto rats.
2000,
Pubmed
Tsuruga,
Identification of novel membrane and secreted proteins upregulated during adipocyte differentiation.
2000,
Pubmed
Wada,
Identification and characterization of galectin-9, a novel beta-galactoside-binding mammalian lectin.
1997,
Pubmed
Wada,
Characterization of mammalian translocase of inner mitochondrial membrane (Tim44) isolated from diabetic newborn mouse kidney.
1998,
Pubmed
Yang,
Identification of a renal-specific oxido-reductase in newborn diabetic mice.
2000,
Pubmed
Zhang,
Collectrin, a collecting duct-specific transmembrane glycoprotein, is a novel homolog of ACE2 and is developmentally regulated in embryonic kidneys.
2001,
Pubmed
,
Xenbase
