Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation.
Cytoplasmic polyadenylation-induced mRNA translation is a hallmark of early animal development. In Xenopus oocytes, where the molecular mechanism has been defined, the core factors that control this process include CPEB, an RNA binding protein whose association with the CPE specifies which mRNAs undergo polyadenylation; CPSF, a multifactor complex that interacts with the near-ubiquitous polyadenylation hexanucleotide AAUAAA; and maskin, a CPEB and eIF4E binding protein whose regulation of initiation is governed by poly(A) tail length. Here, we define two new factors that are essential for polyadenylation. The first is symplekin, a CPEB and CPSF binding protein that serves as a scaffold upon which regulatory factors are assembled. The second is xGLD-2, an unusual poly(A) polymerase that is anchored to CPEB and CPSF even before polyadenylation begins. The identification of these factors has broad implications for biological process that employ polyadenylation-regulated translation, such as gametogenesis, cell cycle progression, and synaptic plasticity.
PubMed ID: 15550246
Article link: Cell.
Grant support: DK32520 NIDDK NIH HHS , F32-GM64872 NIGMS NIH HHS , GM28983 NIGMS NIH HHS , GM46779 NIGMS NIH HHS , HD37267 NICHD NIH HHS
Genes referenced: cpeb1 eif4e papd4 tacc3