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Acetylcholine receptors and concanavalin A-binding sites on cultured Xenopus muscle cells: electrophoresis, diffusion, and aggregation.
Stollberg J
,
Fraser SE
.
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Using digitally analyzed fluorescence videomicroscopy, we have examined the behavior of acetylcholine receptors and concanavalin A binding sites in response to externally applied electric fields. The distributions of these molecules on cultured Xenopus myoballs were used to test a simple model which assumes that electrophoresis and diffusion are the only important processes involved. The model describes the distribution of concanavalin A sites quite well over a fourfold range of electric field strengths; the results suggest an average diffusion constant of approximately 2.3 X 10(-9) cm2/s. At higher electric field strengths, the asymmetry seen is substantially less than that predicted by the model. Acetylcholine receptors subjected to electric fields show distributions substantially different from those predicted on the basis of simple electrophoresis and diffusion, and evidence a marked tendency to aggregate. Our results suggest that this aggregation is due to lateral migration of surface acetylcholine receptors, and is dependent on surface interactions, rather than the rearrangement of microfilaments or microtubules. The data are consistent with a diffusion-trap mechanism of receptor aggregation, and suggest that the event triggering receptor localization is a local increase in the concentration of acetylcholine receptors, or the electrophoretic concentration of some other molecular species. These observations suggest that, whatever mechanism(s) trigger initial clustering events in vivo, the accumulation of acetylcholine receptors can be substantially enhanced by passive, diffusion-mediated aggregation.
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977, Pubmed,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Bloch,
Dispersal and reformation of acetylcholine receptor clusters of cultured rat myotubes treated with inhibitors of energy metabolism.
1979,
Pubmed
Bloch,
The localization of acetylcholine receptor clusters in areas of cell-substrate contact in cultures of rat myotubes.
1980,
Pubmed
Boulter,
Concanavalin A inhibition of alpha-bungarotoxin binding to a nonfusing muscle cell line.
1979,
Pubmed
Brackenbury,
Distinct calcium-independent and calcium-dependent adhesion systems of chicken embryo cells.
1981,
Pubmed
Chao,
Localization of cell membrane components by surface diffusion into a "trap".
1981,
Pubmed
Connolly,
Role of the cytoskeleton in the formation, stabilization, and removal of acetylcholine receptor clusters in cultured muscle cells.
1984,
Pubmed
Davey,
Localization of acetylcholine receptors and cholinesterase on nerve-contacted and noncontacted muscle cells grown in the presence of agents that block action potentials.
1986,
Pubmed
,
Xenbase
Edidin,
Rotational and translational diffusion in membranes.
1974,
Pubmed
Edwards,
A model for the localization of acetylcholine receptors at the muscle endplate.
1976,
Pubmed
Fraser,
Development, maintenance, and modulation of patterned membrane topography: models based on the acetylcholine receptor.
1982,
Pubmed
,
Xenbase
Frye,
The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons.
1970,
Pubmed
Grunwald,
Enzymatic dissection of embryonic cell adhesive mechanisms.
1980,
Pubmed
Henderson,
The absence of calcium blocks impulse-evoked release of acetylcholine but not de novo formation of functional neuromuscular synaptic contacts in culture.
1984,
Pubmed
,
Xenbase
Jaffe,
Electrophoresis along cell membranes.
1977,
Pubmed
Kuromi,
Nerve disperses preexisting acetylcholine receptor clusters prior to induction of receptor accumulation in Xenopus muscle cultures.
1984,
Pubmed
,
Xenbase
Kuromi,
Formation of acetylcholine receptor clusters at neuromuscular junction in Xenopus cultures.
1985,
Pubmed
,
Xenbase
Lin-Liu,
Migration of cell surface concanavalin A receptors in pulsed electric fields.
1984,
Pubmed
,
Xenbase
Marchesi,
Stabilizing infrastructure of cell membranes.
1985,
Pubmed
McDaniel,
The interaction of calcium with gangliosides in bilayer membranes.
1985,
Pubmed
McLaughlin,
The role of electro-osmosis in the electric-field-induced movement of charged macromolecules on the surfaces of cells.
1981,
Pubmed
,
Xenbase
O'Rourke,
Dynamic aspects of retinotectal map formation revealed by a vital-dye fiber-tracing technique.
1986,
Pubmed
,
Xenbase
Orida,
Electrophoretic movement and localisation of acetylcholine receptors in the embryonic muscle cell membrane.
1978,
Pubmed
,
Xenbase
Orida,
On the developmental regulation of acetylcholine receptor mobility in the Xenopus embryonic muscle membrane.
1980,
Pubmed
,
Xenbase
Orida,
Maintenance and dissolution of acetylcholine receptor clusters in the embryonic muscle cell membrane.
1981,
Pubmed
,
Xenbase
Peng,
Polarization of fucoid eggs by steady electrical fields.
1976,
Pubmed
Peng,
Participation of calcium and calmodulin in the formation of acetylcholine receptor clusters.
1984,
Pubmed
,
Xenbase
Poo,
Lateral electrophoresis and diffusion of Concanavalin A receptors in the membrane of embryonic muscle cell.
1978,
Pubmed
,
Xenbase
Poo,
Lateral diffusion of rhodopsin in the photoreceptor membrane.
1974,
Pubmed
Poo,
Electrophoresis of concanavalin A receptors along embryonic muscle cell membrane.
1977,
Pubmed
,
Xenbase
Poo,
In situ electrophoresis of membrane components.
1981,
Pubmed
,
Xenbase
Poo,
Rapid lateral diffusion of functional A Ch receptors in embryonic muscle cell membrane.
1982,
Pubmed
,
Xenbase
Prives,
Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.
1982,
Pubmed
Ravdin,
Fluorescent tetramethyl rhodamine derivatives of alpha-bungarotoxin: preparation, separation, and characterization.
1977,
Pubmed
Role,
On the mechanism of acetylcholine receptor accumulation at newly formed synapses on chick myotubes.
1985,
Pubmed
Schuetze,
Developmental regulation of nicotinic acetylcholine receptors.
1987,
Pubmed
Singer,
The fluid mosaic model of the structure of cell membranes.
1972,
Pubmed
Takeichi,
Functional correlation between cell adhesive properties and some cell surface proteins.
1977,
Pubmed
Weaver,
Diffusion-mediated localization on membrane surfaces.
1983,
Pubmed
Ziskind-Conhaim,
Redistribution of acetylcholine receptors on developing rat myotubes.
1984,
Pubmed
