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Mol Cell Biol
1988 Oct 01;810:4433-40. doi: 10.1128/mcb.8.10.4433-4440.1988.
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Pathway of B1-Alu expression in microinjected oocytes: Xenopus laevis proteins associated with nuclear precursor and processed cytoplasmic RNAs.
Maraia R
,
Zasloff M
,
Plotz P
,
Adeniyi-Jones S
.
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We have previously characterized B1-Alu gene expression by microinjected Xenopus laevis oocytes. The transcription, endonucleolytic processing and its kinetics, nuclear transport kinetics, and subsequent cellular compartmentalization have been described previously (Adeniyi-Jones and Zasloff, Nature 317:81-84, 1985). Briefly, a B1-Alu gene is transcribed by RNA polymerase III to a 210-nucleotide (210nt) primary transcript which is processed to yield 135nt and 75nt RNAs. After processing, the 135nt RNA enters the cytoplasmic compartment, where it remains stable, while the 75nt RNA is degraded. In this report we characterize this pathway further and show that the RNAs involved are complexed with specific X. laevis proteins. The primary transcript was associated with an X. laevis protein of 63 kilodaltons (p63) as well as La, a protein known to be associated with RNA polymerase III transcripts. After processing, the cytoplasmic 135nt RNA remained associated only with the X. laevis p63 in the form of a small ribonucleoprotein. Human autoimmune antibodies were purified by affinity chromatography to X. laevis p63 and used to immunoprecipitate human ribonucleoprotein containing a 63-kilodalton polypeptide and small RNAs. These data suggest that Alu-analogous ribonucleoproteins and their metabolic pathways are conserved across species and provide insight as to their possible functions.
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,
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,
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,
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Anderson,
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,
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Andrews,
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Britten,
Gene regulation for higher cells: a theory.
1969,
Pubmed
Burnette,
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1981,
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Cleveland,
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
1977,
Pubmed
Elder,
Transcriptional analysis of interspersed repetitive polymerase III transcription units in human DNA.
1981,
Pubmed
Fisher,
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1985,
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Gottesfeld,
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,
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Hendrick,
Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
1981,
Pubmed
Hough,
Studies on the repetitive sequence transcripts of Xenopus oocytes.
1972,
Pubmed
,
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Kramerov,
The sequences homologous to major interspersed repeats B1 and B2 of mouse genome are present in mRNA and small cytoplasmic poly(A) + RNA.
1982,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lerner,
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1980,
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Lerner,
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
1979,
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Lerner,
Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus.
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Lerner,
Snurps and scyrps.
1981,
Pubmed
Mathews,
La antigen recognizes and binds to the 3'-oligouridylate tail of a small RNA.
1984,
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Mattioli,
Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus. Description of a cytoplasmic nonribosomal antigen.
1974,
Pubmed
Melton,
Transcription of cloned tRNA genes and the nuclear partitioning of a tRNA precursor.
1979,
Pubmed
,
Xenbase
Olmsted,
Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples.
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Pan,
Structural analysis of interspersed repetitive polymerase III transcription units in human DNA.
1981,
Pubmed
Patton,
Reconstitution of the U1 small nuclear ribonucleoprotein particle.
1987,
Pubmed
Schmid,
The Alu family of dispersed repetitive sequences.
1982,
Pubmed
Siegel,
Each of the activities of signal recognition particle (SRP) is contained within a distinct domain: analysis of biochemical mutants of SRP.
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Siegel,
Elongation arrest is not a prerequisite for secretory protein translocation across the microsomal membrane.
1985,
Pubmed
Siegel,
Removal of the Alu structural domain from signal recognition particle leaves its protein translocation activity intact.
,
Pubmed
Stefano,
Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts.
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Ullu,
Human 7SL RNA consists of a 140 nucleotide middle-repetitive sequence inserted in an alu sequence.
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Walter,
Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum.
1982,
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Walter,
Protein translocation across the endoplasmic reticulum.
1984,
Pubmed
Wolin,
The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.
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Yamagata,
Molecular properties of the Ro/SSA antigen and enzyme-linked immunosorbent assay for quantitation of antibody.
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Yamamoto,
The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA.
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Yang,
A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.
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Young,
Construction and expression in vivo of an internally deleted mouse alpha-fetoprotein gene: presence of a transcribed Alu-like repeat within the first intervening sequence.
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Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
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Pubmed
,
Xenbase