XB-ART-2742Nat Cell Biol December 1, 2004; 6 (12): 1212-20.
Non-canonical Wnt signals are modulated by the Kaiso transcriptional repressor and p120-catenin.
Gastrulation movements are critical for establishing the three principal germ layers and the basic architecture of vertebrate embryos. Although the individual molecules and pathways involved are not clearly understood, non-canonical Wnt signals are known to participate in developmental processes, including planar cell polarity and directed cell rearrangements. Here we demonstrate that the dual-specificity transcriptional repressor Kaiso, first identified in association with p120-catenin, is required for Xenopus gastrulation movements. In addition, depletion of xKaiso results in increased expression of the non-canonical xWnt11, which contributes to the xKaiso knockdown phenotype as it is significantly rescued by dominant-negative Wnt11. We further demonstrate that xWnt11 is a direct gene target of xKaiso and that p120-catenin association relieves xKaiso repression in vivo. Our results indicate that p120-catenin and Kaiso are essential components of a new developmental gene regulatory pathway that controls vertebrate morphogenesis.
PubMed ID: 15543138
Article link: Nat Cell Biol
Genes referenced: ctnnd1 sult4a1 wnt11 wnt11b zbtb33
Morpholinos: sult4a1 MO1 zbtb33 MO1