XB-ART-2763Gene Expr Patterns November 1, 2004; 5 (1): 97-105.
Identification and comparative expression analyses of Daam genes in mouse and Xenopus.
During vertebrate embryogenesis, secreted Wnt molecules regulate cell fates by signaling through the canonical pathway mediated by beta-catenin, and regulate planar cell polarity (PCP) and convergent extension movements through alternative pathways. The phosphoprotein Dishevelled (Dsh/Dvl) is a Wnt signal transducer thought to function in all Wnt signaling pathways. A recently identified member of the Formin family, Daam (Dishevelled--associated activator of morphogenesis), regulates the morphogenetic movements of vertebrate gastrulation in a Wnt-dependent manner through direct interactions with Dsh/Dvl and RhoA. We describe two mouse Daam cDNAs, mDaam1 and mDaam2, which encode proteins characterized by highly conserved formin homology domains and which are expressed in complementary patterns during mouse development. Cross-species comparisons indicate that the expression domains of Xenopus Daam1 (XDaam1) mirror mDaam1 expression. Our results demonstrate that Daams are expressed in tissues known to require Wnts and are consistent with Daams being effectors of Wnt signaling during vertebrate development.
PubMed ID: 15533824
Article link: Gene Expr Patterns
Genes referenced: daam1 dvl1 dvl2 fmn1 rhoa rhoa.2
Article Images: [+] show captions
|Fig. 6. WISH analysis of XDaam1 during Xenopus embryogenesis. Stages of embryos (Nieuwkoop and Faber, 1967) and views are shown above each panel. Sagittal section of embryo in C is shown in D, arrow indicates dorsal lip. Transverse sections of embryo in E are shown in F respectively, arrows indicate neural crest localization. In panels (E),(G) and (H), anterior is to the left.|