Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Nat Immunol. December 1, 2004; 5 (12): 1275-81.

An evolutionarily conserved target motif for immunoglobulin class-switch recombination.

Zarrin AA , Alt FW , Chaudhuri J , Stokes N , Kaushal D , Du Pasquier L , Tian M .

Immunoglobulin H class-switch recombination (CSR) occurs between switch regions and requires transcription and activation-induced cytidine deaminase (AID). Transcription through mammalian switch regions, because of their GC-rich composition, generates stable R-loops, which provide single-stranded DNA substrates for AID. However, we show here that the Xenopus laevis switch region S(mu), which is rich in AT and not prone to form R-loops, can functionally replace a mouse switch region to mediate CSR in vivo. X. laevis S(mu)-mediated CSR occurred mostly in a region of AGCT repeats targeted by the AID-replication protein A complex when transcribed in vitro. We propose that AGCT is a primordial CSR motif that targets AID through a non-R-loop mechanism involving an AID-replication protein A complex.

PubMed ID: 15531884
Article link: Nat Immunol.
Grant support: AI07512 NIAID NIH HHS , AI31541 NIAID NIH HHS

Genes referenced: cda

My Xenbase: [ Log-in / Register ]
version: [3.7.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556